Phylogenomic analysis of Wolbachia genomes from the Darwin Tree of Life biodiversity genomics project.
Phylogenomic analysis of Wolbachia genomes from the Darwin Tree of Life biodiversity genomics project.
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来自达尔文生物生物多样性基因组学项目的沃尔巴氏菌基因组的系统基因组学分析。
DOI：
10.1371/journal.pbio.3001972
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发表时间：
2023-01
期刊：
影响因子：
9.8
通讯作者：
中科院分区：
文献类型：
--
作者：
达尔文生命之树（DToL）项目旨在对在英国和爱尔兰发现的所有描述的陆生和水生真核生物物种进行测序。参考基因组序列由每个靶物种的单个个体生成。除了目标基因组之外，测序样品通常包含来自微生物组、内共生体、寄生虫和其他共生体的遗传物质。Wolbachia内共生细菌存在于多种陆生节肢动物和线虫中，其中超类群A和B在昆虫中最常见。我们确定并组装了110个完整的沃尔巴克氏体基因组从93个宿主物种跨越92个家庭，从368个昆虫物种的DToL项目产生的过滤数据。从15个受感染的物种中，我们组装了一个以上的沃尔巴克氏体基因组，包括同时携带超群A和B感染的个体。不同的昆虫目有不同的感染模式，鳞翅目宿主主要感染超群B，而双翅目和膜翅目的感染主要是A型Wolbachia。除了这些大规模的订单级协会，主机和沃尔巴克氏体reprodenies显示没有（或非常有限）同根生。这表明，在沃尔巴克氏体大流行的进化史中，经常发生宿主转换事件，包括昆虫目之间的转换。虽然超群A和B基因组具有不同的GC%和GC偏斜，并且B基因组具有更大的核心基因集并且倾向于更长，但是噬菌体WO的拷贝的丰度是沃尔巴克氏体基因组大小的强决定因素。挖掘为参考基因组组装生成的原始基因组数据是识别和分析共生体基因组并为其宿主提供更大生态背景的一种强大方式。沃尔巴克氏体是常见的细菌内共生体，在其宿主中操纵生殖生物学。这项研究收集了110个沃尔巴克氏体的基因组，这些基因组与它们的昆虫宿主一起被碰巧测序，发现了丰富多样的宿主操纵位点。
The Darwin Tree of Life (DToL) project aims to sequence all described terrestrial and aquatic eukaryotic species found in Britain and Ireland. Reference genome sequences are generated from single individuals for each target species. In addition to the target genome, sequenced samples often contain genetic material from microbiomes, endosymbionts, parasites, and other cobionts. Wolbachia endosymbiotic bacteria are found in a diversity of terrestrial arthropods and nematodes, with supergroups A and B the most common in insects. We identified and assembled 110 complete Wolbachia genomes from 93 host species spanning 92 families by filtering data from 368 insect species generated by the DToL project. From 15 infected species, we assembled more than one Wolbachia genome, including cases where individuals carried simultaneous supergroup A and B infections. Different insect orders had distinct patterns of infection, with Lepidopteran hosts mostly infected with supergroup B, while infections in Diptera and Hymenoptera were dominated by A-type Wolbachia. Other than these large-scale order-level associations, host and Wolbachia phylogenies revealed no (or very limited) cophylogeny. This points to the occurrence of frequent host switching events, including between insect orders, in the evolutionary history of the Wolbachia pandemic. While supergroup A and B genomes had distinct GC% and GC skew, and B genomes had a larger core gene set and tended to be longer, it was the abundance of copies of bacteriophage WO who was a strong determinant of Wolbachia genome size. Mining raw genome data generated for reference genome assemblies is a robust way of identifying and analysing cobiont genomes and giving greater ecological context for their hosts. Wolbachia are common bacterial endosymbionts that manipulate reproductive biology in their hosts. This study assembles the genomes of 110 Wolbachia coincidentally sequenced alongside their insect hosts, finding a rich diversity of host manipulation loci.
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影响因子：
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作者：
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通讯作者：
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影响因子：
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影响因子：
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