Telophase of the first cleavage is the key stage for optimally inducing mitotic gynogenesis in rice field eel (Monopterus albus)
Telophase of the first cleavage is the key stage for optimally inducing mitotic gynogenesis in rice field eel (Monopterus albus)
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第一次卵裂末期是最佳诱导黄鳝有丝分裂雌核发生的关键阶段
DOI：
10.1016/j.aquaculture.2020.735241
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发表时间：
2020-06
期刊：
影响因子：
4.5
通讯作者：
Wei Hu
中科院分区：
文献类型：
--
作者：
Hongrui Luo;Ke Feng;Ji Chen;Yanlong Song;Mingxi Hou;Yinjun Jiang;Mouyan Jiang;Yongming Li;Binbin Tao;Yaping Wang;Zuoyan Zhu;Wei Hu
黄鳝是我国养殖的重要淡水鱼类。本研究采用热休克（40.5 ± 0.5 °C，3.5min）方法，对经紫外线（UV）照射的鲤鱼（Cyprinus carpio）精子激活的卵子进行有丝分裂雌核发育诱导。最初，使用组织学分析鉴定第一个胚胎细胞周期。然后通过分析二倍体和正常孵化率来确定热激处理的最佳起始时间。采用流式细胞术和核型分析确认倍性。胚胎在70 mpf（受精后分钟）时开始进入前期，第一次卵裂在125 mpf时完成。单倍体和杂种胚在孵化前均死亡。中期前（75 mpf）和末期（100 mpf）的热激处理可诱导染色体加倍，并产生可存活的个体。中期前处理的卵，以60 mpf（相当于原核融合期）热休克处理的二倍体率最高（33.26 ± 4.4%），正常孵化率最高（3.94 ± 0.12%）。末期（100 mpf）处理的卵，二倍体率为100%，最高正常孵化率为26.77%。因此，热激处理诱导黄鳝雌核发育的最佳时间是在第一次有丝分裂的末期。这项技术可能会加速新品种黄鳝的开发。
The rice field eel,Monopterus albus, is an important freshwater fish cultured in China. In this study, mitotic gynogenesis was induced in the rice field eel using heat shock (40.5 ± 0.5 °C for 3.5 min) on eggs activated by ultraviolet (UV) - irradiated sperm of common carp (Cyprinus carpio). Initially, the first embryonic cell cycle was identified using histological analysis. The optimal initiation time for heat shock treatment was then determined by analyzing both the diploid and normal hatching rates. Ploidy was confirmed using flow cytometry and karyotype analyses. Embryos began to enter prophase at 70 mpf (min post-fertilization) and the first cleavage was completed at 125 mpf. Both haploid and hybrid embryos died before hatching. Heat shock treatment before metaphase (75 mpf) and at telophase (100 mpf) can induce chromosome doubling and produce viable individuals. For eggs treated before metaphase, heat shock treatment at 60 mpf, corresponding to the stage of pronuclear fusion, had the highest diploid rate (33.26 ± 4.4%) and normal hatching rate (3.94 ± 0.12%). For eggs treated at telophase (100 mpf), the diploid rate was 100% and the highest normal hatching rate was 26.77%. Therefore, the optimal time to apply heat shock treatment to induce mitotic gynogenesis in the rice field eel was at telophase during the first mitosis process. This technology could potentially accelerate the development of a new breed of rice field eel.
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影响因子：
4.5
作者：
G. M. Weber;Mark A. Hostuttler
通讯作者：
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影响因子：
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影响因子：
4.4
作者：
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作者：
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通讯作者：
M. G. Hussain;D. Penman;B. McAndrew;R. Johnstone
影响因子：
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作者：
Collins, TM;Trexler, JC;Rawlings, TA
通讯作者：
Rawlings, TA