Interfacial degradation of adhesive composite restorations mediated by oral biofilms and mechanical challenge in an extracted tooth model of secondary caries.
Interfacial degradation of adhesive composite restorations mediated by oral biofilms and mechanical challenge in an extracted tooth model of secondary caries.
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在次生龋齿提取的牙齿模型中，由口腔生物膜介导的粘合剂复合修复体的界面降解。
DOI：
10.1016/j.jdent.2017.08.009
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发表时间：
2017-11
影响因子：
4.4
通讯作者：
Rudney J
中科院分区：
文献类型：
--
作者：
Carrera CA;Li Y;Chen R;Aparicio C;Fok A;Rudney J
研究模拟咬合负荷和菌斑生物膜对牙复合材料界面完整性的联合作用，并探讨蔗糖的掺入是否调节了这种作用。在第三磨牙中制备MOD-II类牙列。一半样本（n=27）使用人工口腔环境（ART）进行200，000次机械载荷循环。然后，将两组样本（疲劳和非疲劳）分成三个亚组，用于在以下条件下在CDC反应器中进行测试：无生物膜（对照）、无蔗糖的生物膜（BNS）和蔗糖脉冲的生物膜（BWS）。将BNS和BWS反应器与来自单个噬菌斑供体的多物种接种物一起孵育，而对照反应器没有。BWS反应器每天用蔗糖脉冲五次。连续重复生物膜攻击12周。记录每次运行的pH值。用显微CT和快速断裂试验检测试样的脱矿和承载能力。只有在BWS牙齿中才能检测到脱矿物质。断裂载荷显着降低了伴随存在的生物膜和蔗糖，无论是否施加循环机械载荷。在所有反应器条件下，循环载荷降低了断裂载荷，但降低幅度在统计学上不显著。需要蔗糖脉冲来诱导生物膜介导的粘合剂界面降解。我们已经提出了一个全面的和临床相关的模型，以研究机械负荷和微生物的挑战对牙体充填的界面完整性的影响。
To study the combined effect of simulated occlusal loading and plaque-derived biofilm on the interfacial integrity of dental composite restorations, and to explore whether the effects are modulated by the incorporation of sucrose. MOD-class-II restorations were prepared in third molars. Half of the specimens (n=27) were subjected to 200,000 cycles of mechanical loading using an artificial oral environment (ART). Then, both groups of specimens (fatigued and non-fatigued) were divided into three subgroups for testing in CDC-reactors under the following conditions: no biofilm (Control), biofilm with no sucrose (BNS) and biofilm pulsed with sucrose (BWS). BNS and BWS reactors were incubated with a multispecies inoculum from a single plaque donor whereas the control reactor was not. The BWS reactor was pulsed with sucrose five times a day. The biofilm challenges were repeated sequentially for 12 weeks. pH was recorded for each run. Specimens were examined for demineralization with micro-CT and load capacity by fast fracture test. Demineralization next to the restorations was only detectable in BWS teeth. Fracture loads were significantly reduced by the concomitant presence of biofilm and sucrose, regardless of whether cyclic mechanical loading was applied. Cyclic loading reduced fracture loads under all reactor conditions, but the reduction was not statistically significant. Sucrose pulsing was required to induce biofilm-mediated degradation of the adhesive interface. We have presented a comprehensive and clinically relevant model to study the effects of mechanical loading and microbial challenge on the interfacial integrity of dental restorations.
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影响因子：
5
作者：
Li, Yuping;Carrera, Carola;Chen, Ruoqiong;Li, Jianying;Chen, Yungchung;Lenton, Patricia;Rudney, Joel. D.;Jones, Robert S.;Aparicio, Conrado;Fok, Alex
通讯作者：
Fok, Alex
影响因子：
6.4
作者：
Jorth P;Turner KH;Gumus P;Nizam N;Buduneli N;Whiteley M
通讯作者：
Whiteley M
影响因子：
14
作者：
Lin, BA;Jaffer, F;Santerre, JP
通讯作者：
Santerre, JP
影响因子：
5
作者：
Dejak, Beata;Mlotkowski, Andrzej
通讯作者：
Mlotkowski, Andrzej
影响因子：
14
作者：
Frankenberger, R;Pashley, DH;Tay, FR
通讯作者：
Tay, FR