Structure of an immunodominant epitope of the circumsporozoite surface protein of Plasmodium knowlesi.
Structure of an immunodominant epitope of the circumsporozoite surface protein of Plasmodium knowlesi.
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诺氏疟原虫环子孢子表面蛋白免疫显性表位的结构。
DOI：
10.1021/bi00318a043
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发表时间：
1984
期刊：
影响因子：
2.9
通讯作者：
Nussenzweig,V
中科院分区：
文献类型：
--
作者：
Schlesinger,DH;Cochrane,AH;Gwadz,RW;Godson,GN;Melton,R;Nussenzweig,RS;Nussenzweig,V
David H. Schlesinger,* Alan H. Cochrane, Robert W. Gwadz， G. Nigel Godson, Richard Melton, Ruth S. Nussenzweig和Victor Nussenzweig摘要：先前的研究表明，诺氏疟原虫环孢子子表面（CS）蛋白的免疫优势区包含在一个连续重复的十二肽：Gln-Ala-Gln-Gly-Asp-Gly-Ala-Asn-Ala-Gly-Gln-Pro中。我们在这里表明，ccs蛋白表位与针对完整寄生虫的六种单克隆抗体反应，在该十二肽的合成串联重复中表示。通过合成多肽进一步研究了其中4种抗体的特异性。孢子虫是疟原虫原虫的感染阶段。孢子子的膜上覆盖着一种蛋白[环孢子子表面（CS） 1蛋白]；在Nussenzweig & Nussenweig（1984）中综述]，当寄生虫在无脊椎动物宿主按蚊的唾液腺内成熟时，其合成量相对较大。CS蛋白单克隆抗体Fab片段可在体外重新激活孢子体的感染性并阻止其附着在靶细胞上（Hollingdale et al., 1982）。由于这些原因，有人认为CS蛋白在侵袭宿主肝细胞的过程中起作用
David H. Schlesinger,* Alan H. Cochrane, Robert W. Gwadz, G. Nigel Godson, Richard Melton, Ruth S. Nussenzweig, and Victor Nussenzweig abstract: Previous studies have shown that the immunodominant region of the circumsporozoite surface (CS) protein of Plasmodiumknowlesi is contained within a tandemly re-peated dodecapeptide: Gln-Ala-Gln-Gly-Asp-Gly-Ala-Asn-Ala-Gly-Gln-Pro. We show here that theCS protein epitopes reacting with six monoclonal antibodies raised against the intact parasite are represented in a synthetic tandem repeat of this dodecapeptide. The specificity of four of these anti-bodies was studied further by preparing synthetic peptides¡ Sporozoites are the infective stage of Plasmodium protozoan parasites. The membrane of sporozoites is covered with a protein [circumsporozoite surface (CS) 1 protein; reviewed in Nussenzweig & Nussenweig (1984)] which is synthesized in relatively large amounts when the parasite matures inside the salivary glands of the invertebrate host, Anopheles mosquitoes. Fab fragments of monoclonal antibodies to CS proteins neu-tralize the infectivity of the sporozoites and prevent their attachment to the target cells in vitro (Hollingdale et al., 1982). For these reasons, it has been suggested that the CS protein plays a role in theprocess of invasion of the host liver cells
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固相程序合成的牛甲状旁腺激素氨基末端 1-34 片段的合成、纯化和化学表征。
影响因子：
2.9
作者：
G. Tregear;J. van Rietschoten;R. Sauer;H. Niall;H. Keutmann;J. Potts
通讯作者：
J. Potts
DOI：
10.1073/pnas.79.18.5651
发表时间：
1982-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
COCHRANE, AH;SANTORO, F;NUSSENZWEIG, RS
通讯作者：
NUSSENZWEIG, RS
环子孢子蛋白的单克隆抗体可识别受感染蚊子中的疟原虫种类
影响因子：
64.8
作者：
Fidel Zavala;Fidel Zavala;R. Gwadz;R. Gwadz;F. H. Collins;F. H. Collins;R. Nussenzweig;R. Nussenzweig;Victor Nussenzweig;Victor Nussenzweig
通讯作者：
Victor Nussenzweig
伯氏疟原虫子孢子保护性抗原的抗体可阻止其进入培养细胞。
影响因子：
4.4
作者：
M. Hollingdale;F. Zavala;R. Nussenzweig;V. Nussenzweig
通讯作者：
V. Nussenzweig
影响因子：
64.5
作者：
OZAKI, LS;SVEC, P;GODSON, GN
通讯作者：
GODSON, GN