Changes in rat sperm membrane glycosidase activities and carbohydrate and protein contents associated with epididymal transit.
Changes in rat sperm membrane glycosidase activities and carbohydrate and protein contents associated with epididymal transit.
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与附睾运输相关的大鼠精子膜糖苷酶活性以及碳水化合物和蛋白质含量的变化。
DOI：
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发表时间：
1987
影响因子：
3.6
通讯作者：
Gary J. Killian
中科院分区：
文献类型：
--
作者：
Joseph C. Hall;Gary J. Killian
从附睾头、体和尾回收大鼠精子，并测定糖苷酶活性、总非氨基（中性）碳水化合物和蛋白质含量。荧光法测定精子和膜富集组分中β-葡萄糖苷酶、β-半乳糖苷酶、β-N-乙酰氨基葡萄糖苷酶和β-N-乙酰氨基半乳糖苷酶的活性。除了β-葡萄糖苷酶，基于蛋白质含量的糖苷酶的活动是最大的，在整个精子和膜富集的部分从附睾尾获得。根据精子浓度，糖苷酶活性从附睾头到附睾体逐渐升高，然后从附睾体到附睾尾逐渐下降。基于精子数量的非氨基碳水化合物和蛋白质含量分析表明，区域趋势与糖苷酶活性相似。总非氨基碳水化合物和蛋白质含量在精子体中最高，在精子尾中最低。这些数据表明精子穿过附睾时细胞表面碳水化合物的主要数量变化。增加糖苷酶活性和减少碳水化合物和蛋白质含量之间的膜富集分数的正相关性表明，糖苷酶可能在修改精子表面在附睾运输和成熟过程中发挥重要作用。
Rat spermatozoa were recovered from the caput, corpus, and cauda epididymides and assayed for glycosidase activity, total nonamino (neutral) carbohydrate, and protein content. The activities of beta-glucosidase, beta-galactosidase, beta-N-acetylglucosaminidase, and beta-N-acetylgalactosaminidase were fluorometrically assayed in spermatozoa and membrane-enriched fractions. Except for beta-glucosidase, the activities of the glycosidases based on protein content were greatest in whole sperm and membrane-enriched fractions obtained from the cauda epididymides. Based on sperm concentration, however, glycosidase activities increased proceeding from the caput to the corpus epididymides, then declined from the corpus to the cauda epididymides. Analyses of nonamino carbohydrate and protein content based on sperm number indicated regional trends similar to those of glycosidase activity. Total nonamino carbohydrate and protein content were highest in corpus sperm, and lowest in cauda sperm. These data indicate major quantitative changes in cell surface carbohydrate as spermatozoa traverse the epididymis. A positive correlation for the membrane-enriched fraction between increasing glycosidase activity and decreasing carbohydrate and protein content suggests that glycosidases may play a significant role in modifying the spermatozoon surface during epididymal transit and maturation.
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大鼠尾部附睾精子质膜上成熟相关糖蛋白的表征。
DOI：
--
发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Zeheb,R;Orr,GA
通讯作者：
Orr,GA
附睾运输过程中大鼠精子的表面变化。
影响因子：
3.6
作者：
Olson,GE;Danzo,BJ
通讯作者：
Danzo,BJ
从兔精液中分离β-N-乙酰己糖胺酶及其在受精中的作用。
DOI：
10.1042/bj1910827
发表时间：
1980
期刊：
The Biochemical journal
影响因子：
--
作者：
Farooqui,AA;Srivastava,PN
通讯作者：
Srivastava,PN
男性生殖道中α-乳清蛋白的免疫细胞化学定位。
影响因子：
3.6
作者：
Byers,SW;Qasba,PK;Paulson,HL;Dym,M
通讯作者：
Dym,M