DNA Array Analysis of Changes in Preovulatory Gene Expression in the Rat Ovary1
DNA Array Analysis of Changes in Preovulatory Gene Expression in the Rat Ovary1
复制标题
大鼠卵巢排卵前基因表达变化的 DNA 阵列分析1
作者：
C. Leo;M. Pisarska;A. Hsueh
摘要在围排卵期，哺乳动物卵巢是功能和结构发生剧烈变化的部位，导致卵母细胞成熟、卵泡破裂和黄体形成。在很大程度上，这些过程是不同类型卵巢细胞转录组变化的结果。为了更广泛地了解排卵期卵巢中基因表达的变化，并确定与排卵期事件相关的更多基因，我们使用了最近开发的DNA阵列技术。未成熟雌性心电刺激大鼠在hCG诱导排卵前或排卵后6h处死。提取卵巢总RNA，用于制备放射性标记的cDNA探针，与代表大约600个大鼠基因的DNA阵列杂交。定量分析确定了大量受调控的基因信息，包括几个与细胞外基质降解和类脂/类固醇代谢有关的基因，以前报道是由hCG诱导的。这项筛选还确定了一组候选基因，它们的卵巢表达和促性腺激素调节到目前为止都是未知的。Northern印迹分析证实了其中三个基因的诱导，它们分别编码皮肤脂肪酸结合蛋白、白细胞介素4受体α链和前胶原。此外，原位杂交分析表明，hCG可导致这三个基因在卵泡膜细胞中排他性或优势表达。这些结果表明，DNA阵列可以用于识别排卵期调控基因，从而有助于更详细地了解排卵的分子机制。
Abstract During the periovulatory period, the mammalian ovary is the site of dramatic functional and structural changes, leading to oocyte maturation, follicle rupture, and corpus luteum formation. To a large extent, these processes result from changes in the transcriptome of various ovarian cell types. To develop a broader view of periovulatory changes in gene expression in the ovary and to identify further genes involved in periovulatory events, we used the recently developed DNA array technology. Immature female eCG-primed rats were killed either immediately before or 6 h after ovulation induction with hCG. Total ovarian RNA was isolated and used to prepare radiolabeled cDNA probes, which were hybridized to DNA arrays representing approximately 600 rat genes. Quantitative analysis identified a multitude of regulated gene messages, including several genes involved in extracellular matrix degradation and lipid/steroid metabolism previously reported to be induced by hCG. This screening also identified a group of candidate genes whose ovarian expression and gonadotropin regulation was hitherto unknown. The induction of three of these genes, encoding cutaneous fatty acid-binding protein, the interleukin-4 receptor alpha chain, and prepronociceptin, was confirmed and further characterized by Northern blot analysis. In addition, in situ hybridization analysis showed that hCG administration resulted in exclusive or predominant expression of all three genes in theca cells. These results demonstrate that DNA arrays can be used to identify genes regulated during the periovulatory period, thus contributing to a more detailed understanding of the molecular mechanisms of ovulation.
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DOI：
10.1111/j.1432-1033.1991.tb15736.x
发表时间：
1991
期刊：
European journal of biochemistry
影响因子：
--
作者：
Y. Liu;X. Peng;T. Ny
通讯作者：
Y. Liu;X. Peng;T. Ny
影响因子：
20.3
作者：
J. Richards
通讯作者：
J. Richards
DOI：
10.1073/pnas.94.20.10838
发表时间：
1997-09-30
影响因子：
11.1
作者：
NobenTrauth, N;Shultz, LD;Paul, WE
通讯作者：
Paul, WE
人绒毛膜促性腺激素对卵巢鸟氨酸脱羧酶的调节。
DOI：
10.1152/ajpcell.1987.253.5.c687
发表时间：
1987
期刊：
The American journal of physiology
影响因子：
--
作者：
Sertich,GJ;Persson,L;Pegg,AE
通讯作者：
Pegg,AE