Figla Favors Ovarian Differentiation by Antagonizing Spermatogenesis in a Teleosts, Nile Tilapia (Oreochromis niloticus).
Figla Favors Ovarian Differentiation by Antagonizing Spermatogenesis in a Teleosts, Nile Tilapia (Oreochromis niloticus).
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DOI：
10.1371/journal.pone.0123900
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Zhou L
中科院分区：
文献类型：
--
作者：
Qiu Y;Sun S;Charkraborty T;Wu L;Sun L;Wei J;Nagahama Y;Wang D;Zhou L
Figla(生殖系中的因子，Alpha)是一种女性生殖细胞特有的转录因子，已被证明能激活卵母细胞的遗传等级。已知Figla的异位表达抑制了雄性小鼠的精子发生相关基因。然而，无花果在其他脊椎动物中的潜在作用仍然难以捉摸。本工作旨在鉴定和鉴定无花果在尼罗罗非鱼卵巢发育中的功能相关性。组织分布和个体发育分析表明，无花果罗非鱼基因从孵化后30d开始主要在卵巢中表达。免疫组织化学分析还表明，Figla在早期初级卵母细胞胞浆中有表达。有趣的是，在XY鱼类中，Figla的过度表达导致精子发生的中断，以及睾丸中减数分裂的精母细胞和精子细胞的枯竭。Sycp3(突触膜复合体蛋白3)和PRM(鱼精蛋白)表达的急剧下降表明减数分裂精母细胞和成熟精子的产生受到损害。即使支持细胞(Dmrt1)和间质细胞(STAR和CYP17A1)的标记基因没有受到影响，但hsd3b1的表达和11-KT的产生在无花果转基因睾丸中得到了增强。综上所述，我们的数据表明，鱼绒毛果可能通过拮抗精子发生而在卵巢发育中发挥重要作用。
Figla (factor in the germ line, alpha), a female germ cell-specific transcription factor, had been shown to activate genetic hierarchies in oocytes. The ectopic expression of Figla was known to repress spermatogenesis-associated genes in male mice. However, the potential role of Figla in other vertebrates remains elusive. The present work was aimed to identify and characterize the functional relevance of Figla in the ovarian development of Nile tilapia (Oreochromis niloticus). Tissue distribution and ontogeny analysis revealed that tilapia Figla gene was dominantly expressed in the ovary from 30 days after hatching. Immunohistochemistry analysis also demonstrated that Figla was expressed in the cytoplasm of early primary oocytes. Intriguingly, over-expression of Figla in XY fish resulted in the disruption of spermatogenesis along with the depletion of meiotic spermatocytes and spermatids in testis. Dramatic decline of sycp3 (synaptonemal complex protein 3) and prm (protamine) expression indicates that meiotic spermatocytes and mature sperm production are impaired. Even though Sertoli cell (dmrt1) and Leydig cell (star and cyp17a1) marker genes remained unaffected, hsd3b1 expression and 11-KT production were enhanced in Figla-transgene testis. Taken together, our data suggest that fish Figla might play an essential role in the ovarian development by antagonizing spermatogenesis.
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卵巢基因表达在没有图形的情况下，卵母细胞特异性转录因子。
影响因子：
--
作者：
Joshi, Saurabh;Davies, Holly;Sims, Lauren Porter;Levy, Shawn E.;Dean, Jurrien
通讯作者：
Dean, Jurrien
影响因子：
2.5
作者：
Kobayashi, Tohru;Kajiura-Kobayashi, Hiroko;Nagahama, Yoshitaka
通讯作者：
Nagahama, Yoshitaka
影响因子：
2.5
作者：
Onichtchouk, D;Aduroja, K;Driever, W
通讯作者：
Driever, W
影响因子：
4.8
作者：
Guigon, CJ;Coudouel, N;Magre, S
通讯作者：
Magre, S
DOI：
10.1073/pnas.1007032107
发表时间：
2010-10-05
影响因子：
11.1
作者：
Fujimoto, Takafumi;Nishimura, Toshiya;Arai, Katsutoshi
通讯作者：
Arai, Katsutoshi