Comparison of hyperpolarized (13) C and non-hyperpolarized deuterium MRI approaches for imaging cerebral glucose metabolism at 4.7 T.
Comparison of hyperpolarized (13) C and non-hyperpolarized deuterium MRI approaches for imaging cerebral glucose metabolism at 4.7 T.
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DOI：
10.1002/mrm.28612
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发表时间：
2021-04
影响因子：
3.3
通讯作者：
Garbow JR
中科院分区：
文献类型：
--
作者：
von Morze C;Engelbach JA;Blazey T;Quirk JD;Reed GD;Ippolito JE;Garbow JR
本研究的目的是直接比较两种同位素代谢成像方法，超极化（HP） 13C MRI和氘代谢成像（DMI），在4.7 t时成像大脑糖代谢的特定密切相关的区段，在正常大鼠同一扫描时段连续进行HP 13C和DMI神经成像实验。[1-13C]丙酮酸和[6,6- 2h2]葡萄糖向各自下游代谢产物的局部转化通过光谱成像测量，使用相同的2D CSI序列，并为各自的实验优化参数。为了便于直接比较，开发了一对基本等效的2.5 cm双调谐X / 1H射频表面线圈。为了改善结果，采用多维低秩重构对原始DMI数据进行去噪。惠普的局部转换(1-13C)丙酮酸(1-13C)乳酸和[6 6-2H2]葡萄糖3 3-2H2乳酸和Glx-d(谷氨酸和谷氨酰胺),在老鼠大脑中发现了光谱成像在4.7 t的信噪比和空间分辨率惠普13 c核磁共振是优于DMI但限于一个短的时间窗口,而冗长的DMI收购产生的地图不仅乳酸还Glx生产,尽管相对贫穷的光谱在这场强代谢物之间的歧视。在个体大鼠中，HP [1-13C]乳酸和Glx-d的脑产量呈明显的负相关，并有增加[3,3- 2h2]乳酸的趋势。HP 13C MRI和DMI在4.7 T时都是可行的，并且对葡萄糖代谢的特定部分具有重要的代谢成像潜力。
The purpose of this study was to directly compare two isotopic metabolic imaging approaches, hyperpolarized (HP) 13C MRI and deuterium metabolic imaging (DMI), for imaging specific closely-related segments of cerebral glucose metabolism at 4.7 T. Comparative HP 13C and DMI neuroimaging experiments were conducted consecutively in normal rats during the same scanning session. Localized conversions of [1-13C]pyruvate and [6,6-2H2]glucose to their respective downstream metabolic products were measured by spectroscopic imaging, using an identical 2D CSI sequence with parameters optimized for the respective experiments. To facilitate direct comparison, a pair of substantially-equivalent 2.5-cm double-tuned X / 1H RF surface coils was developed. For improved results, multi-dimensional low-rank reconstruction was applied to denoise the raw DMI data. Localized conversion of HP [1-13C]pyruvate to [1-13C]lactate, and [6,6-2H2]glucose to [3,3-2H2]lactate and Glx-d (glutamate and glutamine), was detected in rat brain by spectroscopic imaging at 4.7 T. The SNR and spatial resolution of HP 13C MRI was superior to DMI but limited to a short time window, while the lengthy DMI acquisition yielded maps of not only lactate but also Glx production, albeit with relatively poor spectral discrimination between metabolites at this field strength. Across the individual rats, there was an apparent inverse correlation between cerebral production of HP [1-13C]lactate and Glx-d, along with a trend toward increased [3,3-2H2]lactate. HP 13C MRI and DMI are both feasible at 4.7 T and have significant potential for metabolic imaging of specific segments of glucose metabolism.
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影响因子：
2.5
作者：
Hu, Simon;Larson, Peder E. Z.;Vigneron, Daniel B.
通讯作者：
Vigneron, Daniel B.
影响因子：
4.6
作者：
Brender, Jeffrey R.;Kishimoto, Shun;Krishna, Murali C.
通讯作者：
Krishna, Murali C.
影响因子：
3.3
作者：
Josan, Sonal;Hurd, Ralph;Billingsley, Kelvin;Senadheera, Lasitha;Park, Jae Mo;Yen, Yi-Fen;Pfefferbaum, Adolf;Spielman, Daniel;Mayer, Dirk
通讯作者：
Mayer, Dirk
影响因子：
13.6
作者：
De Feyter HM;Behar KL;Corbin ZA;Fulbright RK;Brown PB;McIntyre S;Nixon TW;Rothman DL;de Graaf RA
通讯作者：
de Graaf RA
DOI：
10.1073/pnas.0601319103
发表时间：
2006-07-25
影响因子：
11.1
作者：
Golman, Klaes;in't Zandt, Rene;Thaning, Mikkel
通讯作者：
Thaning, Mikkel