Effects of isoflurane anesthesia on hyperpolarized (13)C metabolic measurements in rat brain.
Effects of isoflurane anesthesia on hyperpolarized (13)C metabolic measurements in rat brain.
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DOI：
10.1002/mrm.24532
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发表时间：
2013-10
影响因子：
3.3
通讯作者：
Mayer, Dirk
中科院分区：
文献类型：
--
作者：
Josan, Sonal;Hurd, Ralph;Billingsley, Kelvin;Senadheera, Lasitha;Park, Jae Mo;Yen, Yi-Fen;Pfefferbaum, Adolf;Spielman, Daniel;Mayer, Dirk
已知常用的麻醉剂如异氟烷是有效的脑血管扩张剂，据报道脑血流量和脑血容量呈剂量依赖性增加。尽管异氟烷在超极化 13C 临床前研究中得到广泛使用，但对其代谢测量影响的定量评估仍然有限。这项工作研究了异氟烷麻醉剂量对大鼠脑中 [1-13C]丙酮酸和 2-酮[1-13C]异己酸超极化 13C MR 代谢测量的影响。动态2D和3D螺旋化学位移成像用于获取大鼠脑以及肾脏和肝脏在推注超极化[1-13C]丙酮酸盐或2-酮[1-13C]异己酸盐后的代谢图像。研究了“低剂量”与“高剂量”异氟烷对大脑代谢水平和表观转化率的影响。发现脑底物信号水平以及因此代谢物与底物的比率和表观转化率显着取决于异氟醚剂量，而代谢产物的信号水平及其比率，例如碳酸氢盐/乳酸盐对异氟醚水平基本上不敏感。在肾脏或肝脏中没有观察到丙酮酸对异氟烷的明显依赖性。这项研究强调了仔细关注麻醉对大脑超极化 13C 代谢成像代谢测量的影响的重要性。
Commonly used anesthetic agents such as isoflurane are known to be potent cerebral vasodilators, with reported dose-dependent increase in cerebral blood flow and cerebral blood volume. Despite the widespread use of isoflurane in hyperpolarized 13C pre-clinical research studies, a quantitative assessment of its effect on metabolic measurements is limited. This work investigates the effect of isoflurane anesthesia dose on hyperpolarized 13C MR metabolic measurements in rat brain for [1-13C]pyruvate and 2-keto[1-13C]isocaproate. Dynamic 2D and 3D spiral chemical shift imaging was used to acquire metabolic images of rat brain as well as kidney and liver following bolus injections of hyperpolarized [1-13C]pyruvate or 2-keto[1-13C]isocaproate. The impact of a ‘low dose’ vs. a ‘high dose’ of isoflurane on cerebral metabolite levels and apparent conversion rates was examined. The cerebral substrate signal levels, and hence the metabolite-to-substrate ratios and apparent conversion rates, were found to depend markedly on isoflurane dose, while signal levels of metabolic products and their ratios, e.g. bicarbonate/lactate, were largely insensitive to isoflurane levels. No obvious dependence on isoflurane was observed in kidney or liver for pyruvate. This study highlights the importance of careful attention to the effects of anesthesia on the metabolic measures for hyperpolarized 13C metabolic imaging in brain.
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影响因子：
8.8
作者：
CHI, OZ;ANWAR, M;WEISS, HR
通讯作者：
WEISS, HR
影响因子：
3.3
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Alsop, David C.
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82.9
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通讯作者：
Brindle, Kevin M.
影响因子：
3.3
作者：
Day, Sam E.;Kettunen, Mikko I.;Brindle, Kevin M.
通讯作者：
Brindle, Kevin M.
影响因子：
8.8
作者：
Lenz, C;Rebel, A;Waschke, KF
通讯作者：
Waschke, KF