Hydroalcoholic Extract from Inflorescences of Achyrocline satureioides (Compositae) Ameliorates Dextran Sulphate Sodium-Induced Colitis in Mice by Attenuation in the Production of Inflammatory Cytokines and Oxidative Mediators.
Hydroalcoholic Extract from Inflorescences of Achyrocline satureioides (Compositae) Ameliorates Dextran Sulphate Sodium-Induced Colitis in Mice by Attenuation in the Production of Inflammatory Cytokines and Oxidative Mediators.
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DOI：
10.1155/2016/3475356
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Faloni de Andrade S
中科院分区：
文献类型：
--
作者：
da Silva LM;Farias JA;Boeing T;Somensi LB;Beber AP;Cury BJ;Santin JR;Faloni de Andrade S
Achyrocline satureioides是一种南美草药，用于治疗炎症和胃肠道疾病。本研究评价了香茶菜花序水醇提取物（HEAS）对葡聚糖硫酸钠（DSS）诱导的小鼠结肠炎的肠道抗炎作用。小鼠经口给予溶媒、5-氨基水杨酸（100 mg/kg）或HEAS（1 - 100 mg/kg）。评价结肠炎的临床体征和结肠组织病理学参数，沿着测定结肠中还原型谷胱甘肽和脂质过氧化氢（LOOH）水平、超氧化物歧化酶（SOD）和髓过氧化物酶（MPO）活性。测量细胞因子（TNF、IL-4、IL-6和IL-10）的结肠含量。此外，测定了提取物对脂多糖（LPS）刺激的巨噬细胞释放一氧化氮（NO）和二苯基苦肼水平的影响。结肠组织中的粘蛋白水平和SOD活性，以及LOOH、MPO、TNF和IL-6积累通过HEAS给药而正常化。此外，提取物引起结肠中IL-4和IL-10水平的增加。HEAS可抑制巨噬细胞释放NO，并证实其具有清除NO的活性。总之，这些结果表明，从A.结论：番泻叶可用于IBD的治疗。此外，这项工作证实了普遍使用的A。在炎症性疾病中的satureioides。
Achyrocline satureioides is a South American herb used to treat inflammatory and gastrointestinal diseases. This study evaluated intestinal anti-inflammatory effects of the hydroalcoholic extract of inflorescences of satureioides (HEAS) in dextran sulfate sodium (DSS) induced colitis in mice. Mice were orally treated with vehicle, 5-aminosalicylic acid (100 mg/kg), or HEAS (1–100 mg/kg). Clinical signs of colitis and colonic histopathological parameters were evaluated, along with the determination of levels of reduced glutathione and lipid hydroperoxide (LOOH), the superoxide dismutase (SOD), and myeloperoxidase (MPO) activity in colon. The colonic content of cytokines (TNF, IL-4, IL-6, and IL-10) was measured. Additionally, the effects of the extract on nitric oxide (NO) release by lipopolysaccharide (LPS) stimulated macrophages and diphenylpicrylhydrazyl levels were determined. Mucin levels and SOD activity, as well as the LOOH, MPO, TNF, and IL-6 accumulation in colon tissues, were normalized by the HEAS administration. In addition, the extract elicited an increase in IL-4 and IL-10 levels in colon. NO release by macrophages was inhibited by HEAS and its scavenger activity was confirmed. Together these results suggest that preparations obtained from inflorescences from A. satureioides could be used in treatment for IBD. Besides, this work corroborates the popular use of A. satureioides in inflammatory disorders.
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