Effects of subacute exposure to 1,2-dichloroethane on mouse behavior and the related mechanisms
Effects of subacute exposure to 1,2-dichloroethane on mouse behavior and the related mechanisms
复制标题
1,2-二氯乙烷亚急性暴露对小鼠行为的影响及相关机制
DOI：
10.1177/0960327112470270
复制
发表时间：
2013-09
期刊：
影响因子：
--
通讯作者：
Jin, Y. P.
中科院分区：
文献类型：
--
作者：
Gao, L.;Li, G.;Lv, X.;Jin, Y. P.
本研究旨在探讨亚急性暴露于1,2-二氯乙烷（1,2- dce）对小鼠行为的影响及其相关机制，重点探讨脑内氧化应激和氨基酸神经递质的改变。采用开场试验观察小鼠行为。用比色法测定血清一氧化氮（NO）、丙二醛（MDA）和非蛋白巯基（NPSH）水平以及诱导型一氧化氮合酶（iNOS）和超氧化物歧化酶（SOD）活性。采用高效液相色谱法测定谷氨酸（Glu）、天冬氨酸（Asp）和γ -氨基丁酸（GABA）的含量。在0.45 g/m3和0.9 g/m3的1,2- dce处理的小鼠中，发现运动和探索活动减少，焦虑增加。相比之下，0.225 g/m3 1,2- dce处理小鼠的兴奋性增加。在1,2- dce处理的小鼠中，发现代偿性抗氧化状态和脑内NOS活性和NO水平升高。此外，与对照组相比，1,2- dce处理小鼠的Glu含量和0.9 g/m3 1,2- dce处理小鼠的GABA含量增加，而0.225 g/m3 1,2- dce处理小鼠的GABA含量显著降低。综上所述，我们的研究结果表明，亚急性暴露于1,2- dce后，小鼠的行为会受到干扰，大脑中氨基酸神经递质的变化可能与行为影响有关。
The aim of this study was to explore the effects of subacute exposure to 1,2-dichloroethane (1,2-DCE) on mouse behavior and the related mechanisms focusing on alteration of oxidative stress and amino acid neurotransmitters in the brain. Mouse behavior was examined by open field test. Levels of nitric oxide (NO), malondialdehyde (MDA) and nonprotein sulfhydryl (NPSH) and activity of inducible nitric oxide synthase (iNOS) and superoxide dismutase (SOD) were determined by colorimetric method. Contents of glutamate (Glu), aspartate (Asp) and gamma-aminobutyric acid (GABA) were evaluated by high-performance liquid chromatography. Reduced locomotor and exploratory activities and increased anxiety were found in 0.45 and 0.9 g/m3 1,2-DCE-treated mice. In contrast, increased excitability was found in 0.225 g/m3 1,2-DCE-treated mice. Compensatory antioxidant status and increased NOS activity and NO level in the brain were found in 1,2-DCE-treated mice. Moreover, Glu contents in 1,2-DCE-treated mice and GABA contents in 0.9 g/m3 1,2-DCE-treated mice increased, whereas GABA contents in 0.225 g/m3 1,2-DCE-treated mice decreased significantly compared with control. Taken together, our results suggested that mouse behavior could be disturbed by subacute exposure to 1,2-DCE, and the changes of amino acid neurotransmitter in the brain might be related to the behavioral effects.
登录
查看更多内容
影响因子：
3.6
作者：
Cao, Junran;Belluzzi, James D.;Loughlin, Sandra E.;Dao, Jasmin M.;Chen, YiLing;Leslie, Frances M.
通讯作者：
Leslie, Frances M.
影响因子：
2.8
作者：
Juanwen Zhang;G. Lv;Ying Zhao
通讯作者：
Juanwen Zhang;G. Lv;Ying Zhao
DOI：
--
发表时间：
1988-05
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
--
作者：
R. W. D'souza;W. R. Francis;M. Andersen
通讯作者：
R. W. D'souza;W. R. Francis;M. Andersen
影响因子：
2.7
作者：
F. Zhan;W. Zheng;Lian Liu;L. Kong
通讯作者：
F. Zhan;W. Zheng;Lian Liu;L. Kong
影响因子：
5.3
作者：
Hopper, Rachel A.;Garthwaite, John
通讯作者：
Garthwaite, John