Combination of active components enhances the efficacy of Prunella in prevention and treatment of lung cancer.
Combination of active components enhances the efficacy of Prunella in prevention and treatment of lung cancer.
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DOI：
10.3390/molecules15117893
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发表时间：
2010-11-04
期刊：
影响因子：
--
通讯作者：
Shi F
中科院分区：
文献类型：
--
作者：
Feng L;Jia XB;Jiang J;Zhu MM;Chen Y;Tan XB;Shi F
夏枯草提取物在预防和治疗肺癌方面的功效已归因于不同的成分。本研究提出了夏枯草抗肿瘤活性的“活性成分组合模型”假说。比较不同产地夏枯草提取物的体内外药效，并测定其对荷瘤小鼠血清TNF-α活性的影响。采用高效液相色谱法（HPLC）对提取物进行分析，鉴定出26个共有峰。用聚类分析方法对不同产地的夏枯草样品进行了分类，夏枯草和夏枯草均为中国夏枯草。亳州和南京的P. asiaticaNakai，进一步划分为不同的类群。HPLC分析的6个峰非常相似，分别鉴定为咖啡酸、迷迭香酸、芦丁、槲皮素、油酸和熊果酸。亳州夏枯草和南京夏枯草中这些化合物的总比例分别为1.0：14.7：3.9：1.0：4.4：1.4和1.0：14.8：4.0：0.8：5.6：1.8。采用大孔树脂分离纯化夏枯草中的总三萜和总酚，并进行活性研究。结果表明，总三萜和总酚具有抗肺癌活性，二者联合作用可显著增强活性。此外，与总三萜或总酚相比，该组合还显著增加了TNF-α含量。结果表明，夏枯草抗肺癌的作用是由多种成分以最佳比例共同作用的结果。
The efficacy of Prunella extracts in the prevention and treatment of lung cancer has been attributed to different components. In this study, an "active components combination model" hypothesis was proposed to explain the anti-tumor activity of Prunella. The efficacy of Prunella extracts from different regions was compared in vitro and in vivo, and the TNF-α activity in serum of tumor-bearing mice was also evaluated. High performance liquid chromatography (HPLC) was used to analyze the extracts and identify 26 common peaks. Prunella samples from different regions were classified by the cluster analysis method; both P. vulgaris L. from Bozhou and P. asiatica Nakai from Nanjing, which had the highest activities, were further divided into different classes. Six peaks from the HPLC analysis were very similar, and were identified as caffeic acid, rosmarinic acid, rutin, quercetin, oleanolic acid and ursolic acid. The total ratio of these compounds in Prunella from Bozhou and Nanjing were 1.0:14.7:3.9:1.0:4.4:1.4 and 1.0:14.8:4.0:0.8:5.6:1.8, respectively. Total triterpenes and total phenols in Prunella were separated by macroporous resin purification for activity studies. The results showed that total triterpenes and total phenols had anti-lung cancer activity and their combination significantly enhanced the activity. In addition, the combination also significantly increased the TNF-α content compared to total triterpenes or total phenols. The results indicated that the efficacy of Prunella against lung cancer was attributable to multiple components acting at an optimal ratio.
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影响因子：
4.3
作者：
Han, Eun Hee;Choi, Jae Ho;Jeong, Hye Gwang
通讯作者：
Jeong, Hye Gwang
影响因子：
7.2
作者：
Psotová, J;Kolár, M;Ulrichová, J
通讯作者：
Ulrichová, J
DOI：
10.1016/j.bbrc.2004.04.139
发表时间：
2004-06-11
影响因子：
3.1
作者：
Gaudineau, C;Beckerman, R;Auclair, K
通讯作者：
Auclair, K
DOI：
10.1016/0165-1218(88)90093-6
发表时间：
1988-02-01
期刊：
MUTATION RESEARCH
影响因子：
--
作者：
LEE, H;LIN, JY
通讯作者：
LIN, JY
影响因子：
3.6
作者：
Wan, LX;Cao, DL;Pizzorno, G
通讯作者：
Pizzorno, G