Anterior and posterior corneal stroma elasticity after corneal collagen crosslinking treatment.
Anterior and posterior corneal stroma elasticity after corneal collagen crosslinking treatment.
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DOI：
10.1016/j.exer.2013.07.028
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发表时间：
2013-11
影响因子：
3.4
通讯作者：
Ziebarth, Noel M.
中科院分区：
文献类型：
--
作者：
Dias, Janice;Diakonis, Vasilios F.;Kankariya, Vardhaman P.;Yoo, Sonia H.;Ziebarth, Noel M.
本项目的目的是通过压痕使用原子力显微镜（AFM）评估角膜胶原交联（CXL）治疗后的人尸体眼前后角膜基质弹性。本研究包括24只人尸眼（12对），并分为2组（每组6对）。在两组中，左眼（OS）作为对照（不进行核黄素或CXL治疗），右眼（OD）经历CXL治疗（核黄素预处理30分钟，随后暴露于3 mW/cm 2的紫外光30分钟）。在第1组中，通过手动剥离约50μm的角膜基质（包括Bowman膜）暴露前基质。在第2组中，通过剥离包括Bowman膜在内的前50%的角膜基质来暴露后基质。在治疗眼的情况下，在交联治疗后进行分层。在所有眼睛中，使用AFM通过压痕来量化基质弹性。未处理对照眼的前角膜（组1）的杨氏弹性模量为245.9±209.1kPa（范围：82.3 - 530.8 kPa），CXL处理眼的杨氏弹性模量为467.8±373.2kPa（范围：157.4 - 1126 kPa）。未治疗对照眼的后角膜（第2组）杨氏模量为100.2±61.9kPa（范围：28.1 - 162.6 kPa），CXL治疗眼的杨氏模量为66.0±31.8kPa（范围：31.3 - 101.7 kPa）。CXL治疗后前基质的杨氏模量显著增加（p=0.024），而CXL治疗后基质的杨氏模量无显著差异（p=0.170）。对照组和CXL治疗组的前基质硬度均高于后基质（分别为p=0.077和p=0.023）。我们的研究结果表明，CXL治疗后前角膜基质的硬度似乎显着增加，而后基质似乎不受CXL的影响。
The purpose of this project was to assess anterior and posterior corneal stromal elasticity after corneal collagen cross linking (CXL) treatment in human cadaver eyes using Atomic Force Microscopy (AFM) through indentation. Twenty four human cadaver eyes (12 pairs) were included in this study and divided into 2 groups (6 pairs per group). In both groups, the left eye (OS) served as a control (no riboflavin or CXL treatment was performed) and the right eye (OD) underwent CXL treatment (30 minutes of riboflavin pretreatment followed by 30 minutes of exposure to 3mW/cm2 of ultraviolet light). In group 1, the anterior stroma was exposed by manual delamination of approximately 50μm of the corneal stroma including Bowman’s membrane. In group 2, the posterior stroma was exposed by delamination of the anterior 50% of the corneal stroma including Bowman’s membrane. Delamination was performed after crosslinking treatment in the case of the treated eyes. In all eyes, the stromal elasticity was quantified using AFM through indentation. Young’s modulus of elasticity for the anterior cornea (group 1) was 245.9±209.1kPa (range: 82.3 - 530.8 kPa) for the untreated control eyes, and 467.8±373.2kPa (range: 157.4 – 1126 kPa) for the CXL treated eyes. Young’s modulus for the posterior cornea (group 2) was 100.2±61.9kPa (range: 28.1 - 162.6 kPa) for the untreated control eyes and 66.0±31.8kPa (range: 31.3 - 101.7 kPa) for the CXL treated eyes. Young’s modulus of the anterior stroma significantly increased after CXL treatment (p=0.024), whereas the posterior stroma did not demonstrate a significant difference in Young’s modulus after CXL treatment (p=0.170). The anterior stroma was stiffer than the posterior stroma for both the control and CXL treatment groups (p=0.077 and p=0.023, respectively). Our findings demonstrate that stiffness of the anterior corneal stroma after CXL treatment seems to increase significantly, while the posterior stroma does not seem to be affected by CXL.
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影响因子：
13.7
作者：
Kymionis, George D.;Grentzelos, Michael A.;Panagopoulou, Sophia I.
通讯作者：
Panagopoulou, Sophia I.
影响因子：
3.4
作者：
Roy, Abhijit Sinha;Rocha, Karol M.;Dupps, William J., Jr.
通讯作者：
Dupps, William J., Jr.
影响因子：
2.8
作者：
Borja, D;Manns, F;Parel, JM
通讯作者：
Parel, JM
影响因子：
3.5
作者：
Fernández, DC;Niazy, AM;Juhasz, T
通讯作者：
Juhasz, T
DOI：
10.1073/pnas.91.3.836
发表时间：
1994-02-01
影响因子：
11.1
作者：
KARRASCH, S;HEGERL, R;ENGEL, A
通讯作者：
ENGEL, A