Effects of atrial natriuretic factor, sodium nitroprusside, and acetylcholine on cyclic GMP levels and relaxation in rat aorta.
Effects of atrial natriuretic factor, sodium nitroprusside, and acetylcholine on cyclic GMP levels and relaxation in rat aorta.
复制标题
心钠素、硝普钠和乙酰胆碱对大鼠主动脉环 GMP 水平和舒张的影响。
DOI：
10.1016/0014-2999(85)90694-6
复制
发表时间：
1985
影响因子：
5
通讯作者：
Murad,F
中科院分区：
文献类型：
--
作者：
Rapoport,RM;Waldman,SA;Schwartz,K;Winquist,RJ;Murad,F
本研究旨在探讨内皮依赖性血管扩张剂、乙酰胆碱、硝基血管扩张剂、硝普钠和心房利钠因子(心钠素II)升高大鼠胸主动脉环鸟苷酸(CGMP)水平并诱导其松弛的机制。亚甲蓝抑制硝普钠和乙酰胆碱引起的环GMP水平升高和松弛，但不抑制心房肽II的松弛。氰化物抑制对这三种血管扩张剂的松弛，但仅抑制硝普钠和乙酰胆碱对环GMP水平的影响。还原剂硼氢化钠、二硫苏糖醇、蔗糖和异丙肾上腺素均能抑制硝普钠和乙酰胆碱引起的环化GMP水平升高，而心房肽II引起的环化GMP水平升高不受硼氢化钠、蔗糖和异丙肾上腺素的影响。降压剂对血管扩张剂引起的松弛的影响很难解释，因为它们具有非特异性的收缩和松弛特性。已知的抑制Na+，K+泵和对内皮依赖性血管扩张剂和硝普钠的松弛的药物和程序，包括哇巴因、无K+、无镁和低Na+的Krebs-Ringer碳酸氢盐溶液，都部分抑制了对心房肽II的松弛。这些结果表明，与硝普钠和乙酰胆碱不同，心钠素诱导的cGMP水平升高的机制不涉及自由基的形成，也不涉及与血红素的相互作用。此外，硝普钠、乙酰胆碱和心钠素反应形成的环状GMP通过一种共同的机制介导松弛，这种松弛可能被导致膜去极化的药物和程序所拮抗。
The purpose of this study was to investigate the mechanisms whereby an endothelium-dependent vasodilator, acetylcholine, a nitrovasodilator, sodium nitroprusside and atrial natriuretic factor (atriopeptin II), elevate cyclic GMP levels and induce relaxation in rat thoracic aorta. Methylene blue inhibited the elevated cyclic GMP levels and relaxation due to sodium nitroprusside and acetylcholine, but not those to atriopeptin II. Cyanide inhibited relaxations to all three vasodilators, but inhibited the elevated cyclic GMP levels in response to only nitroprusside and acetylcholine. The reducing agents sodium borohydride, dithiothreitol, sucrose and isoproterenol all inhibited the elevated cyclic GMP levels due to nitroprusside and acetylcholine, while the increased cyclic GMP levels with atriopeptin II were unaffected by sodium borohydride, sucrose and isoproterenol. The effects of the reducing agents on relaxation induced by the vasodilators were difficult to interpret due to their nonspecific contractile and relaxant properties. Agents and procedures known to inhibit the Na+, K+-pump and relaxation to endothelium-dependent vasodilators and nitroprusside, including ouabain, K+-free, Mg2+-free and low Na+Krebs-Ringer bicarbonate solution, all partially inhibited relaxations to atriopeptin II. Relaxations to atriopeptin II were also inhibited in tissues contracted with KCl. The present results suggest that the mechanism of atrial natriuretic factor-induced increased cyclic GMP levels, in contrast to that of nitroprusside and acetylcholine, does not involve the formation of free radicals, a reducible species or interaction with heme. Furthermore, the cyclic GMP formed in response to nitroprusside, acetylcholine and atrial natriuretic factor mediates relaxation through a common mechanism that may be functionally antagonized by agents and procedures which result in membrane depolarization.
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心房钠尿因子引起内皮依赖性松弛并激活血管平滑肌中的颗粒鸟苷酸环化酶。
DOI：
10.1073/pnas.81.23.7661
发表时间：
1984
影响因子：
11.1
作者：
Winquist,RJ;Faison,EP;Waldman,SA;Schwartz,K;Murad,F;Rapoport,RM
通讯作者：
Rapoport,RM
心钠素在体外引起血管舒张，这不依赖于内皮细胞的存在。
影响因子：
5
作者：
R. Scivoletto;M. Carvalho
通讯作者：
M. Carvalho
DOI：
10.1016/s0021-9258(18)33448-3
发表时间：
1982-11
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
M. Wolin;K. S. Wood;L. Ignarro
通讯作者：
M. Wolin;K. S. Wood;L. Ignarro
硝普钠和其他平滑肌松弛剂增加大鼠输精管中的环 GMP 水平
影响因子：
64.8
作者：
K. Schultz;K. Schultz;G. Schultz
通讯作者：
G. Schultz
DOI：
--
发表时间：
1983
期刊：
影响因子：
--
作者：
G. Thibault;Raúl García;M. Cantin;A. Genest
通讯作者：
A. Genest