Analysis of glucose metabolism by (18)F-FDG-PET imaging and glucose transporter expression in a mouse model of intracerebral hemorrhage.
Analysis of glucose metabolism by (18)F-FDG-PET imaging and glucose transporter expression in a mouse model of intracerebral hemorrhage.
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通过（18）F-FDG-PET成像和葡萄糖转运蛋白表达在脑出血的小鼠模型中分析葡萄糖代谢。
DOI：
10.1038/s41598-021-90216-4
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发表时间：
2021-05-25
影响因子：
4.6
通讯作者：
Koehler RC
中科院分区：
文献类型：
--
作者：
Han X;Ren H;Nandi A;Fan X;Koehler RC
脑出血后脑糖代谢与葡萄糖转运蛋白表达的关系尚不清楚。很少有研究使用正电子发射断层扫描（PET）来研究啮齿动物脑出血后的脑糖代谢。在这项研究中，我们通过在小鼠胃内注射胶原酶来产生脑出血，以研究18f -氟-2-脱氧-d -葡萄糖(FDG)-PET图像中的葡萄糖代谢变化是否与葡萄糖转运蛋白（GLUTs）的表达有关。在脑出血后第1天和第3天，同侧纹状体表现出明显的低代谢。然而，在第7天和第14天，同侧纹状体的葡萄糖代谢明显高于对侧纹状体。脑出血后对侧半球未出现高代谢。定性免疫荧光和Western blot检测显示，脑出血后第1、3天同侧纹状体GLUT1表达下降，第21天逐渐恢复到基线水平。脑出血后18F-FDG摄取与GLUT1的表达有关，而与GLUT3或GLUT5无关。我们的数据表明，同侧脑葡萄糖代谢在脑出血后早期下降，在晚期逐渐增加。PET成像上18F-FDG摄取的变化与同侧纹状体中GLUT1的表达有关。
The relationship between cerebral glucose metabolism and glucose transporter expression after intracerebral hemorrhage (ICH) is unclear. Few studies have used positron emission tomography (PET) to explore cerebral glucose metabolism after ICH in rodents. In this study, we produced ICH in mice with an intrastriatal injection of collagenase to investigate whether glucose metabolic changes in 18F-fluoro-2-deoxy-D-glucose (FDG)-PET images are associated with expression of glucose transporters (GLUTs) over time. On days 1 and 3 after ICH, the ipsilateral striatum exhibited significant hypometabolism. However, by days 7 and 14, glucose metabolism was significantly higher in the ipsilateral striatum than in the contralateral striatum. The contralateral hemisphere did not show hypermetabolism at any time after ICH. Qualitative immunofluorescence and Western blotting indicated that the expression of GLUT1 in ipsilateral striatum decreased on days 1 and 3 after ICH and gradually returned to baseline by day 21. The 18F-FDG uptake after ICH was associated with expression of GLUT1 but not GLUT3 or GLUT5. Our data suggest that ipsilateral cerebral glucose metabolism decreases in the early stage after ICH and increases progressively in the late stage. Changes in 18F-FDG uptake on PET imaging are associated with the expression of GLUT1 in the ipsilateral striatum.
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影响因子：
3.4
作者：
Li Q;Weiland A;Chen X;Lan X;Han X;Durham F;Liu X;Wan J;Ziai WC;Hanley DF;Wang J
通讯作者：
Wang J
影响因子：
2.7
作者：
Allen, Angela;Messier, Claude
通讯作者：
Messier, Claude
影响因子：
2.4
作者：
Kim, Yong Wook;Kim, Hyoung Seop;An, Young-Sil
通讯作者：
An, Young-Sil
DOI：
10.1161/atvbaha.117.309184
发表时间：
2017-09-01
影响因子：
8.7
作者：
Fidler, Trevor P.;Middleton, Elizabeth A.;Abel, E. Dale
通讯作者：
Abel, E. Dale
影响因子：
8.3
作者：
HEISS, WD;EMUNDS, HG;HERHOLZ, K
通讯作者：
HERHOLZ, K