Association of Zinc deficiency, oxidative stress and increased double-stranded DNA breaks in globozoospermic infertile patients and its implication for the assisted reproductive technique.
Association of Zinc deficiency, oxidative stress and increased double-stranded DNA breaks in globozoospermic infertile patients and its implication for the assisted reproductive technique.
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球精症不孕患者缺锌、氧化应激和双链 DNA 断裂增加的关联及其对辅助生殖技术的意义
DOI：
10.21037/tau-20-1116
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发表时间：
2021-03
影响因子：
2
通讯作者：
Xu W
中科院分区：
文献类型：
--
作者：
Huang L;Yao G;Huang G;Jiang C;Li L;Liao L;Yuan G;Shang L;Xu W
背景：精子DNA片段化及其对辅助生殖技术（ART）结局的不良影响在以前已有报道。然而，锌元素与球形精子症不育患者DNA损伤和卵胞浆内单精子注射（ICSI）结局的关系尚不清楚。方法采用火焰原子吸收分光光度计和超氧化物歧化酶（SOD）测定法，分别检测球形精子症不育患者和生育志愿者精浆中铜、铁、锰、锌和超氧化物歧化酶（SOD）活性水平。采用精子染色质分散试验（SCD）和彗星试验检测两组精液DNA损伤情况。此外，还用苯胺蓝染色法观察了精子核的成熟情况。结果球形精子症不育患者精浆锌水平和SOD活性均低于正常生育者，双链断裂DFI（DSB-DFI）显著高于正常生育者。SOD抗氧化能力不足和低锌水平可能是导致精子DNA损伤的氧化应激机制之一。缺锌还可能影响球形精子发生过程中染色质的稳定性，使其更易受到氧化损伤。结论严重的DSB和精子锌元素缺乏导致的抗氧化功能低下可能是导致球形精子症ICSI失败的原因。
Background Sperm DNA fragmentation and its adverse impact on outcomes of assisted reproductive techniques (ART) in globozoospermic infertile patients has been previously reported. However, the association of Zinc element with DNA damage and intracytoplasmic sperm injection (ICSI) outcome in globozoospermic infertile patients remains unclear. Methods Using flame atomic absorption spectrophotometer and superoxide dismutase (SOD) assay, the levels of Cu, Fe, Mn, Zn and SOD activities in seminal plasma from both globozoospermic infertile patients and fertile volunteers were tested respectively. Using sperm chromatin dispersion (SCD) test and Comet assay, the DNA damages in their semen samples from the two groups was detected. In addition, using Aniline Blue staining, their sperm nucleus maturations were also examined. Results The levels of seminal Zinc and SOD activities were lower in the globozoospermic infertile patients and the double-stranded break DFI (DSB-DFI) were significantly higher than that in the fertile controls. Antioxidative insufficiency of SOD with a low Zn level might be responsible for oxidative stress, which may lead to DNA damage in globozoospermic spermatozoa. Zn deficiency might also have influence on the chromatin stabilization of globozoospermic spermatozoa during spermiogenesis, causing its more vulnerable to oxidative attack. Conclusions Serious DSBs in globozoospermia and antioxidative insufficiency due to Zinc element deficiency in spermatozoa might be responsible for the failure of ICSI in globozoospermia.
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影响因子：
4
作者：
Enciso, Maria;Sarasa, Jonas;Gosalvez, Jaime
通讯作者：
Gosalvez, Jaime
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影响因子：
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作者：
Han F;Liu C;Zhang L;Chen M;Zhou Y;Qin Y;Wang Y;Chen M;Duo S;Cui X;Bao S;Gao F
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影响因子：
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作者：
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Chalchi, Mohammad Javad
DOI：
10.1016/j.jtemb.2018.08.005
发表时间：
2018-01-01
影响因子：
3.5
作者：
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通讯作者：
Massanyi, Peter