Fus3p and Kss1p control G1 arrest in Saccharomyces cerevisiae through a balance of distinct arrest and proliferative functions that operate in parallel with Far1p.
Fus3p and Kss1p control G1 arrest in Saccharomyces cerevisiae through a balance of distinct arrest and proliferative functions that operate in parallel with Far1p.
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Fus3p 和 Kss1p 通过与 Far1p 并行运作的不同阻滞和增殖功能的平衡来控制酿酒酵母中的 G1 阻滞。
作者：
Vera Cherkasova;David M. Lyons;E. Elion
在酿酒酵母中，交配信息素激活两种 MAP 激酶 (MAPK) Fus3p 和 Kss1p，在交配前诱导 G1 期停滞。 Fus3p 已知可通过激活 Far1p 促进 G1 停滞，Far1p 抑制三种 Clnp/Cdc28p 激酶。为了分析 Fus3p 和 Kss1p 对独立于 Far1p 的 G1 停滞的贡献，我们构建了 far1 CLN 菌株，该菌株因交配 MAP 激酶途径激活增加而经历 G1 停滞。我们发现 Fus3p 和 Kss1p 都通过与 Far1p 并行运行的多个功能来控制 G1 停滞。 Fus3p 和 Kss1p 共同通过阻止 Cln3p/Cdc28p 激酶激活的机制来抑制 G1/S 细胞周期蛋白基因（CLN1、CLN2、CLB5）的转录，从而共同促进 G1 期停滞。此外，Fus3p 和 Kss1p 通过重叠和不同的功能来抵消 G1 停滞。 Fus3p 和 Kss1p 一起增加促进出芽的 CLN3 和 PCL2 基因的表达，Kss1p 抑制 MAP 激酶级联。引人注目的是，Fus3p 通过一种新功能促进增殖，该功能与 Ste12p 活性降低或 Cln2p/Cdc28p 激酶水平升高无关。遗传分析表明 Fus3p 通过激活 Mcm1p 转录因子来促进增殖，从而上调 G1 期的众多基因。因此，Fus3p 和 Kss1p 通过抑制 Cdc28p 机制的停滞功能和绕过这种抑制的增殖功能的平衡来控制 G1 停滞。
In Saccharomyces cerevisiae, mating pheromones activate two MAP kinases (MAPKs), Fus3p and Kss1p, to induce G1 arrest prior to mating. Fus3p is known to promote G1 arrest by activating Far1p, which inhibits three Clnp/Cdc28p kinases. To analyze the contribution of Fus3p and Kss1p to G1 arrest that is independent of Far1p, we constructed far1 CLN strains that undergo G1 arrest from increased activation of the mating MAP kinase pathway. We find that Fus3p and Kss1p both control G1 arrest through multiple functions that operate in parallel with Far1p. Fus3p and Kss1p together promote G1 arrest by repressing transcription of G1/S cyclin genes (CLN1, CLN2, CLB5) by a mechanism that blocks their activation by Cln3p/Cdc28p kinase. In addition, Fus3p and Kss1p counteract G1 arrest through overlapping and distinct functions. Fus3p and Kss1p together increase the expression of CLN3 and PCL2 genes that promote budding, and Kss1p inhibits the MAP kinase cascade. Strikingly, Fus3p promotes proliferation by a novel function that is not linked to reduced Ste12p activity or increased levels of Cln2p/Cdc28p kinase. Genetic analysis suggests that Fus3p promotes proliferation through activation of Mcm1p transcription factor that upregulates numerous genes in G1 phase. Thus, Fus3p and Kss1p control G1 arrest through a balance of arrest functions that inhibit the Cdc28p machinery and proliferative functions that bypass this inhibition.
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POG1 是一种新型酵母基因，可通过 G1 细胞周期蛋白 CLN2 促进信息素停滞的恢复。
影响因子：
3.3
作者：
Leza,MA;Elion,EA
通讯作者：
Elion,EA
DOI：
10.1073/pnas.88.21.9392
发表时间：
1991-11-01
影响因子：
11.1
作者：
ELION, EA;BRILL, JA;FINK, GR
通讯作者：
FINK, GR
DOI：
10.1073/pnas.88.23.10966
发表时间：
1991-12-01
影响因子：
11.1
作者：
ELBLE, R;TYE, BK
通讯作者：
TYE, BK
影响因子：
11.4
作者：
NASH, R;TOKIWA, G;FUTCHER, AB
通讯作者：
FUTCHER, AB
影响因子：
10.5
作者：
McInerny, CJ;Partridge, JF;Breeden, LL
通讯作者：
Breeden, LL