Chitosan-based scaffolds for the support of smooth muscle constructs in intestinal tissue engineering.
Chitosan-based scaffolds for the support of smooth muscle constructs in intestinal tissue engineering.
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DOI：
10.1016/j.biomaterials.2012.03.051
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发表时间：
2012-06
期刊：
影响因子：
14
通讯作者：
Bitar, Khalil N.
中科院分区：
文献类型：
--
作者：
Zakhem, Elie;Raghavan, Shreya;Gilmont, Robert R.;Bitar, Khalil N.
肠组织工程是一个新兴的领域，由于不断增长的需求，肠延长和替代程序继发于大规模切除肠。在这里，我们展示了壳聚糖/胶原支架作为3D基质的潜在用途，以支持生物工程化的环形肌肉结构保持其生理功能。通过在壳聚糖包被的培养板上培养兔结肠环形平滑肌细胞，研究壳聚糖的生物相容性。细胞保持其梭形形态并保留其平滑肌表型标记。我们制造了由壳聚糖和胶原蛋白以1：1的比例组成的具有中心开口的管状支架。使用接种有RCSMC的基于纤维蛋白的水凝胶对同心对齐的3D环形肌肉构建体进行生物工程化。将构建体放置在支架周围2周，之后将其取下并测试其生理功能。肌肉结构响应于乙酰胆碱（Ach）和氯化钾（KCl）收缩，并且响应于血管活性肠肽（VIP）松弛。这些结果表明，壳聚糖是一种生物材料，可能适用于肠组织工程的应用。
Intestinal tissue engineering is an emerging field due to a growing demand for intestinal lengthening and replacement procedures secondary to massive resections of the bowel. Here, we demonstrate the potential use of a chitosan/collagen scaffold as a 3D matrix to support the bioengineered circular muscle constructs maintain their physiological functionality. We investigated the biocompatibility of chitosan by growing rabbit colonic circular smooth muscle cells (RCSMCs) on chitosan-coated plates. The cells maintained their spindle-like morphology and preserved their smooth muscle phenotypic markers. We manufactured tubular scaffolds with central openings composed of chitosan and collagen in a 1:1 ratio. Concentrically-aligned 3D circular muscle constructs were bioengineered using fibrin-based hydrogel seeded with RCSMCs. The constructs were placed around the scaffold for 2 weeks, after which they were taken off and tested for their physiological functionality. The muscle constructs contracted in response to Acetylcholine (Ach) and potassium chloride (KCl) and they relaxed in response to vasoactive intestinal peptide (VIP). These results demonstrate that chitosan is a biomaterial possibly suitable for intestinal tissue engineering applications.
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