Virological and serological findings in Rousettus aegyptiacus experimentally inoculated with vero cells-adapted hogan strain of Marburg virus.
Virological and serological findings in Rousettus aegyptiacus experimentally inoculated with vero cells-adapted hogan strain of Marburg virus.
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DOI：
10.1371/journal.pone.0045479
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Kemp A
中科院分区：
文献类型：
--
作者：
Paweska JT;Jansen van Vuren P;Masumu J;Leman PA;Grobbelaar AA;Birkhead M;Clift S;Swanepoel R;Kemp A
埃及果蝠Rousettus aegyptiacus目前被认为是马尔堡病毒(MARV)的潜在宿主。然而，传播方式、病毒复制水平、组织嗜性和病毒脱落模式仍有待描述。通过不同的接种途径，将人工饲养的埃及斑潜蝇(Regyptiacus)，包括成年雄性、雌性和幼仔，暴露于MARV。经鼻、口联合接种的9只蝙蝠的血液、组织、粪便和尿液均为阴性，至接种后21d仍未检出抗体。21只蝙蝠经腹膜/皮下联合接种后，在接种后第2~9天检测到病毒血症和不同组织中MARV的存在，第9~21天检测到免疫球蛋白G抗体。在皮下接种的3只蝙蝠中，在第5天和第8天(实验终止)检测到病毒血症，并从不同的器官分离病毒。3个实验组的尿液、粪便和口腔拭子中均未检出MARV。然而，在可能有助于水平或垂直传播的组织中，如肺、肠、肾、膀胱、唾液腺和女性生殖道中检测到该病毒。持续至少5天的病毒血症也可促进MARV通过吸血节肢动物的机械传播和通过直接接触感染的血液感染易感脊椎动物宿主。所有蝙蝠临床正常，尸检未发现肉眼病变。这项工作证实了埃及伊蚊对MARV感染的易感性，不分性别和年龄，并为建立蝙蝠丝状病毒实验模型做出了贡献。需要进一步的研究来揭示MARV的传播方式，并调查埃及斑潜蝇作为宿主的假定作用。
The Egyptian fruit bat, Rousettus aegyptiacus, is currently regarded as a potential reservoir host for Marburg virus (MARV). However, the modes of transmission, the level of viral replication, tissue tropism and viral shedding pattern remains to be described. Captive-bred R. aegyptiacus, including adult males, females and pups were exposed to MARV by different inoculation routes. Blood, tissues, feces and urine from 9 bats inoculated by combination of nasal and oral routes were all negative for the virus and ELISA IgG antibody could not be demonstrated for up to 21 days post inoculation (p.i.). In 21 bats inoculated by a combination of intraperitoneal/subcutaneous route, viremia and the presence of MARV in different tissues was detected on days 2–9 p.i., and IgG antibody on days 9–21 p.i. In 3 bats inoculated subcutaneously, viremia was detected on days 5 and 8 (termination of experiment), with virus isolation from different organs. MARV could not be detected in urine, feces or oral swabs in any of the 3 experimental groups. However, it was detected in tissues which might contribute to horizontal or vertical transmission, e.g. lung, intestines, kidney, bladder, salivary glands, and female reproductive tract. Viremia lasting at least 5 days could also facilitate MARV mechanical transmission by blood sucking arthropods and infections of susceptible vertebrate hosts by direct contact with infected blood. All bats were clinically normal and no gross pathology was identified on post mortem examination. This work confirms the susceptibility of R. aegyptiacus to infection with MARV irrespective of sex and age and contributes to establishing a bat-filovirus experimental model. Further studies are required to uncover the mode of MARV transmission, and to investigate the putative role of R. aegyptiacus as a reservoir host.
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影响因子：
3.4
作者：
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通讯作者：
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影响因子：
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