Fluoroquinolone resistance mechanism of clinical isolates and selected mutants of Pasteurella multocida from bovine respiratory disease in China.
Fluoroquinolone resistance mechanism of clinical isolates and selected mutants of Pasteurella multocida from bovine respiratory disease in China.
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我国牛呼吸道疾病多杀性巴氏杆菌临床分离株及突变株对氟喹诺酮类药物的耐药机制
DOI：
10.1292/jvms.14-0240
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发表时间：
2014-12
期刊：
影响因子：
--
通讯作者：
Ma HX
中科院分区：
文献类型：
--
作者：
Kong LC;Gao D;Gao YH;Liu SM;Ma HX
对23株多杀性巴氏杆菌（Pasteurella multocida，Pm）的最低抑菌浓度（minimum inhibitory concentration，MIC）、突变预防浓度（mutation prevention concentration，MPC）以及喹诺酮类药物耐药决定区（quinolone resistance determining region，QRDR）突变对氟喹诺酮类药物（环丙沙星、恩诺沙星和奥比沙星）敏感性的影响进行了研究。氟喹诺酮敏感分离株（MIC ≤0.25 µg/ml，9株分离株）无QRDR突变，其各自的MPC较低。氟喹诺酮中间分离株（MIC =0.5 µg/ml，14株分离株）存在QRDR突变（Asp 87突变为Asn或Ala 84突变为gyrA中的Pro），其各自的MPC较高（4-32 µg/ml）。第一步突变体（n=5）和实验室来源的高度耐药氟喹诺酮突变体（n=5）也具有QRDR突变。在存在外排抑制剂的情况下，氟喹诺酮类药物对穆氏杆菌衍生菌株的MIC降低。结果表明，Pm对氟喹诺酮类药物的耐药主要是由于gyrA和parC的多个靶基因突变以及外排泵基因的过度表达所致。
The minimum inhibitory concentrations (MICs), mutation prevention concentrations (MPCs) and contribution of quinolone resistance-determining region (QRDR) mutations to fluoroquinolone (ciprofloxacin, enrofloxacin and orbifloxacin) susceptibility in 23 Pasteurella multocida (Pm) isolates were investigated. Fluoroquinolone-susceptible isolates (MICs ≤0.25 µg/ml, 9 isolates) had no QRDR mutations, and their respective MPCs were low. Fluoroquinolone-intermediate isolates (MICs=0.5 µg/ml, 14 isolates) had QRDR mutations (Asp87 to Asn or Ala84 to Pro in gyrA), and their respective MPCs were high (4–32 µg/ml). First-step mutants (n=5) and laboratory-derived highly resistant fluoroquinolone mutants (n=5) also had QRDR mutations. The MICs of fluoroquinolones for mutant-derived strains were decreased in the presence of efflux inhibitors. The results indicated that the fluoroquinolone resistance of Pm is mainly due to multiple target gene mutations in gyrA and parC and the overexpression of efflux pump genes.
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影响因子：
5.2
作者：
Michael, Geovana Brenner;Kadlec, Kristina;Schwarz, Stefan
通讯作者：
Schwarz, Stefan
DOI：
10.1016/s0732-8893(01)00335-2
发表时间：
2002-02-01
影响因子：
2.9
作者：
Ribera, A;Jurado, A;Vila, J
通讯作者：
Vila, J
影响因子：
3.3
作者：
Katsuda, K.;Hoshinoo, K.;Mikami, O.
通讯作者：
Mikami, O.
影响因子：
2.8
作者：
Chen, Yen-Ping;Tsao, Ming-Yang;Tsai, Hsiang-Jung
通讯作者：
Tsai, Hsiang-Jung
影响因子：
9.4
作者：
Townsend, KM;Frost, AJ;Dawkins, HJS
通讯作者：
Dawkins, HJS