Antioxidant and anticholinesterase investigations of Rumex hastatus D. Don: potential effectiveness in oxidative stress and neurological disorders.
Antioxidant and anticholinesterase investigations of Rumex hastatus D. Don: potential effectiveness in oxidative stress and neurological disorders.
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DOI：
10.1186/s40659-015-0010-2
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发表时间：
2015-03-26
影响因子：
6.7
通讯作者：
Imran M
中科院分区：
文献类型：
--
作者：
Ahmad S;Ullah F;Ayaz M;Sadiq A;Imran M
鲁梅克斯物种传统上用于治疗神经系统疾病，包括头痛，偏头痛，抑郁症，瘫痪等几个物种已被科学验证的抗氧化剂和抗胆碱酯酶的潜力。本研究旨在对戟叶酸模（Rumex hastatus D.唐粗甲醇提取物，随后的馏分，皂苷和黄酮类化合物的乙酰胆碱酯酶，丁酰胆碱酯酶抑制和各种抗氧化活性，以验证其在神经系统疾病的民间用途。戟叶酸模粗甲醇提取物（Rh. Cr），后续馏分;正己烷（Rh. Hex）、氯仿（Rh. Chf）、乙酸乙酯（Rh. EtAc）、水性级分（Rh. Aq）、粗皂苷（Rh. Sp）和类黄酮（Rh. F1）对乙酰胆碱酯酶（AChE）和丁酰胆碱酯酶（BChE）在不同浓度（125、250、500、1000 μg/mL）下的作用。Rh的抗氧化潜力。Sp和Rh。使用DPPH、H2 O2和ABTS自由基清除测定在62.5、125、250、500、1000 μg/mL下评价Fl。所有供试品均表现出浓度依赖性的胆碱酯酶抑制活性和自由基清除活性。Rh. Sp和Rh。Fl是最突出的，即，在最高浓度下，IC 50分别为135和20 μg/mL，分别为81.67 ± 0.88和91.62 ± 1.67。所有随后的级分都表现出中度至高度的AChE抑制，即，Rh. Cr、Rh。十六进制，Rh。Chf，Rh。EtAc和Rh。Aq的IC 50分别为218、1420、75、115和1210 μg/mL。类似地，针对BChE，各种植物提取物，即，Rh. Sp、Rh. Fl、Rh. Cr、Rh。十六进制，Rh。Chf，Rh。EtAc和Rh。Aq的IC 50分别为165、175、265、890、92、115和220 μg/mL。在DPPH自由基清除试验中，Rh. Sp和Rh。F1显示出与阳性对照相当的结果，即，在1 mg/mL浓度下，清除率分别为63.34 ± 0.98和76.93 ± 1.13%（IC 50 312和104 μg/mL）。Rh所表现出的ABTS自由基清除潜力百分比。Sp和Rh。Fl（1000 μg/mL）分别为82.58 ± 0.52和88.25 ± 0.67，IC_（50）分别为18和9 μg/mL。同样，在H2 O2清除试验中，Rh. Sp和Rh。Fl分别表现出175和275 μg/mL的IC 50。Rh具有较强的抗胆碱酯酶活性和抗氧化活性。Sp、Rh. F1和R. hastatus支持民族医药用途，推荐R. hastatus作为治疗AD和神经退行性疾病的可能的补救措施。
Rumex species are traditionally used for the treatment of neurological disorders including headache, migraine, depression, paralysis etc. Several species have been scientifically validated for antioxidant and anticholinestrase potentials. This study aims to investigate Rumex hastatus D. Don crude methanolic extract, subsequent fractions, saponins and flavonoids for acetylcholinestrase, butyrylcholinestrase inhibition and diverse antioxidant activities to validate its folkloric uses in neurological disorders. Rumex hastatus crude methanolic extract (Rh. Cr), subsequent fractions; n-hexane (Rh. Hex), chloroform (Rh. Chf), ethyl acetate (Rh. EtAc), aqueous fraction (Rh. Aq), crude saponins (Rh. Sp) and flavonoids (Rh. Fl) were investigated against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) at various concentrations (125, 250, 500, 1000 μg/mL) using Ellman’s spectrophotometric analysis. Antioxidant potentials of Rh. Sp and Rh. Fl were evaluated using DPPH, H2O2 and ABTS free radical scavenging assays at 62.5, 125, 250, 500, 1000 μg/mL. All the test samples showed concentration dependent cholinesterase inhibition and radicals scavenging activity. The AChE inhibition potential of Rh. Sp and Rh. Fl were most prominent i.e., 81.67 ± 0.88 and 91.62 ± 1.67 at highest concentration with IC50 135 and 20 μg/mL respectively. All the subsequent fractions exhibited moderate to high AChE inhibition i.e., Rh. Cr, Rh. Hex, Rh. Chf, Rh. EtAc and Rh. Aq showed IC50 218, 1420, 75, 115 and 1210 μg/mL respectively. Similarly, against BChE various plant extracts i.e., Rh. Sp, Rh. Fl, Rh. Cr, Rh. Hex, Rh. Chf, Rh. EtAc and Rh. Aq resulted IC50 165, 175, 265, 890, 92, 115 and 220 μg/mL respectively. In DPPH free radical scavenging assay, Rh. Sp and Rh. Fl showed comparable results with the positive control i.e., 63.34 ± 0.98 and 76.93 ± 1.13% scavenging at 1 mg/mL concentration (IC50 312 and 104 μg/mL) respectively. The percent ABTS radical scavenging potential exhibited by Rh. Sp and Rh. Fl (1000 μg/mL) were 82.58 ± 0.52 and 88.25 ± 0.67 with IC50 18 and 9 μg/mL respectively. Similarly in H2O2 scavenging assay, the Rh. Sp and Rh. Fl exhibited IC50 175 and 275 μg/mL respectively. The strong anticholinesterase and antioxidant activities of Rh. Sp, Rh. Fl and various fractions of R. hastatus support the purported ethnomedicinal uses and recommend R. hastatus as a possible remedy for the treatment of AD and neurodegenerative disorders.
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影响因子：
--
作者：
Ayaz M;Junaid M;Ahmed J;Ullah F;Sadiq A;Ahmad S;Imran M
通讯作者：
Imran M
影响因子：
4.8
作者：
Aarsland, Dag;Beyer, Mona K.;Kurz, Martin W.
通讯作者：
Kurz, Martin W.
影响因子：
6.1
作者：
Kerem, Z;Bilkis, I;Sivan, U
通讯作者：
Sivan, U
影响因子：
2.7
作者：
Papandreou, Magdalini A.;Dimakopoulou, Andriana;Lamari, Fotini N.
通讯作者：
Lamari, Fotini N.
影响因子：
2.6
作者：
Perry, EK;Pickering, AT;Perry, NSL
通讯作者：
Perry, NSL