Meiotic nuclear movements in fission yeast are regulated by the transcription factor Mei4 downstream of a Cds1-dependent replication checkpoint pathway.
Meiotic nuclear movements in fission yeast are regulated by the transcription factor Mei4 downstream of a Cds1-dependent replication checkpoint pathway.
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DOI：
10.1111/gtc.12207
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发表时间：
2015-03
期刊：
影响因子：
--
通讯作者：
Hiraoka Y
中科院分区：
文献类型：
--
作者：
Ruan K;Yamamoto TG;Asakawa H;Chikashige Y;Masukata H;Haraguchi T;Hiraoka Y
在减数分裂中，裂殖酵母细胞核呈现出细长的形态，在细胞内来回移动；这些核运动在减数分裂核分裂之前持续大约 2 小时。减数分裂 DNA 复制发生在核运动的早期阶段，随后是减数分裂前期。在这里，我们报告说，在减数分裂 DNA 复制缺陷的突变体中，核运动的持续时间显着延长至 4 至 5 小时。我们发现这种延长是由 Cds1 依赖性复制检查点引起的，该检查点抑制编码减数分裂特异性转录因子的 mei4+ 基因的表达。在没有 Mei4 的情况下，核运动持续超过 8 小时。相比之下，Mei4 的过量产生将核运动的终止加速到大约 30 分钟。这些结果表明，Mei4 参与核运动的终止，并且 Mei4 介导的调控途径将 DNA 复制检查点与核运动的终止联系起来。
In meiosis, the fission yeast nucleus displays an elongated morphology, moving back and forth within the cell; these nuclear movements continue for approximately 2 h before meiotic nuclear divisions. Meiotic DNA replication occurs in an early phase of the nuclear movements and is followed by meiotic prophase. Here we report that in mutants deficient in meiotic DNA replication, the duration of nuclear movements is strikingly prolonged to four to 5 h. We found that this prolongation was caused by the Cds1-dependent replication checkpoint, which represses expression of the mei4+ gene encoding a meiosis-specific transcription factor. In the absence of Mei4, nuclear movements persisted for more than 8 h. In contrast, overproduction of Mei4 accelerated termination of nuclear movements to approximately 30 min. These results show that Mei4 is involved in the termination of nuclear movements and that Mei4-mediated regulatory pathways link a DNA replication checkpoint to the termination of nuclear movements.
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DOI：
10.1083/jcb.200605074
发表时间：
2006-08-14
期刊：
The Journal of cell biology
影响因子：
--
作者：
Ding DQ;Sakurai N;Katou Y;Itoh T;Shirahige K;Haraguchi T;Hiraoka Y
通讯作者：
Hiraoka Y
影响因子：
2.1
作者：
Chikashige, Y;Kurokawa, R;Hiraoka, Y
通讯作者：
Hiraoka, Y
影响因子：
9.2
作者：
Funaya, Charlotta;Samarasinghe, Shivanthi;Pruggnaller, Sabine;Ohta, Midori;Connolly, Yvonne;Mueller, Jan;Murakami, Hiroshi;Grallert, Agnes;Yamamoto, Masayuki;Smith, Duncan;Antony, Claude;Tanaka, Kayoko
通讯作者：
Tanaka, Kayoko
影响因子：
64.5
作者：
Chikashige, Y;Tsutsumi, C;Hiraoka, Y
通讯作者：
Hiraoka, Y
影响因子：
56.9
作者：
CHIKASHIGE, Y;DING, DQ;HIRAOKA, Y
通讯作者：
HIRAOKA, Y