Photonic crystal enhanced fluorescence emission and blinking suppression for single quantum dot digital resolution biosensing.
Photonic crystal enhanced fluorescence emission and blinking suppression for single quantum dot digital resolution biosensing.
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DOI：
10.1038/s41467-022-32387-w
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发表时间：
2022-08-08
影响因子：
16.6
通讯作者：
Cunningham, Brian T.
中科院分区：
文献类型：
--
作者：
Xiong, Yanyu;Huang, Qinglan;Canady, Taylor D.;Barya, Priyash;Liu, Shengyan;Arogundade, Opeyemi H.;Race, Caitlin M.;Che, Congnyu;Wang, Xiaojing;Zhou, Lifeng;Wang, Xing;Kohli, Manish;Smith, Andrew M.;Cunningham, Brian T.
虽然纳米级量子发射体是用于测量生物分子相互作用的有效标签，但是它们对于需要单个单元观测的应用的效用受限于对大数值孔径（NA）物镜、荧光不透明度和由全向发射导致的差的光子收集效率的要求。在这里，我们报告了近3000倍的信号增强通过增强激发，高度定向提取，量子效率的提高，并通过光子晶体（PC）表面闪烁抑制乘法效应实现。该方法实现了具有高信噪比的单量子点（QD）灵敏度，即使在使用低NA透镜和廉价的光学设置时。PC的闪烁抑制能力将QD的接通时间从15%提高到85%，从而改善信号的不稳定性。我们开发了一种检测癌症相关miRNA生物标志物的方法，具有单分子分辨率，单碱基突变选择性和10阿摩尔检测限。此外，我们观察到差异表面运动轨迹的量子点时，它们的表面附着的严格性改变了一个癌症特异性的miRNA序列中的一个单一的碱基。纳米级发射器可用于测量生物分子相互作用，但受到弱信号的限制。在这里，作者使用光子晶体表面进行3000倍的信号增强，实现单发射器灵敏度和延长的接通时间，并展示其在miRNA生物标志物传感中的应用。
While nanoscale quantum emitters are effective tags for measuring biomolecular interactions, their utilities for applications that demand single-unit observations are limited by the requirements for large numerical aperture (NA) objectives, fluorescence intermittency, and poor photon collection efficiency resulted from omnidirectional emission. Here, we report a nearly 3000-fold signal enhancement achieved through multiplicative effects of enhanced excitation, highly directional extraction, quantum efficiency improvement, and blinking suppression through a photonic crystal (PC) surface. The approach achieves single quantum dot (QD) sensitivity with high signal-to-noise ratio, even when using a low-NA lens and an inexpensive optical setup. The blinking suppression capability of the PC improves the QDs on-time from 15% to 85% ameliorating signal intermittency. We developed an assay for cancer-associated miRNA biomarkers with single-molecule resolution, single-base mutation selectivity, and 10-attomolar detection limit. Additionally, we observed differential surface motion trajectories of QDs when their surface attachment stringency is altered by changing a single base in a cancer-specific miRNA sequence. Nanoscale emitters are useful for measuring biomolecular interactions, but are limited by weak signals. Here, the authors use a photonic crystal surface for 3000-fold signal enhancement, achieving single emitter sensitivity with extended on-time, and demonstrate its application in miRNA biomarker sensing.
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影响因子：
4.6
作者：
Dong L;Meng Y;Sui Z;Wang J;Wu L;Fu B
通讯作者：
Fu B
影响因子：
3.8
作者：
Ganesh, Nikhil;Block, Ian D.;Cunningham, Brian T.
通讯作者：
Cunningham, Brian T.
影响因子：
16.6
作者：
Cai S;Pataillot-Meakin T;Shibakawa A;Ren R;Bevan CL;Ladame S;Ivanov AP;Edel JB
通讯作者：
Edel JB
DOI：
10.1007/s11468-013-9660-5
发表时间：
2014
期刊：
Plasmonics (Norwell, Mass.)
影响因子：
--
作者：
Bauch M;Toma K;Toma M;Zhang Q;Dostalek J
通讯作者：
Dostalek J
影响因子：
4.3
作者：
Kim, Taekyung;Paik, Joonki
通讯作者：
Paik, Joonki