Lactobacillus casei abundance is associated with profound shifts in the infant gut microbiome.
Lactobacillus casei abundance is associated with profound shifts in the infant gut microbiome.
复制标题
乳杆菌的丰度与婴儿肠道微生物组的深刻变化有关。
DOI：
10.1371/journal.pone.0008745
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发表时间：
2010-01-18
期刊：
影响因子：
3.7
通讯作者：
Lynch SV
中科院分区：
文献类型：
--
作者：
Cox MJ;Huang YJ;Fujimura KE;Liu JT;McKean M;Boushey HA;Segal MR;Brodie EL;Cabana MD;Lynch SV
在婴儿出生后的第一年，微生物在婴儿肠道的定植对于平衡免疫反应的发展至关重要。新生儿胃肠道微生物群的早期改变与年龄较大的儿童随后发生哮喘和特应性有关。在这里，我们描述了高分辨率的文化独立的分析粪便样本从6个月大的婴儿喂养每天补充干酪乳杆菌亚种。鼠李糖乳杆菌（LGG）或安慰剂在婴儿益生菌补充剂（TIPS）的双盲，随机试验。使用高密度微阵列，16S rRNA PhyloChip，细菌群落组成进行了检查，并比较了LGG丰度高或低的婴儿的微生物组合。具有高丰度LGG的社区表现出促进包括许多其他已知益生菌物种在内的益生菌遗传聚类分类群，并且在其社区成员的分布中显着更均匀。从生物学上讲，这些方面是对病原体的干扰和生长更具抵抗力的群落的特征。PhyloChip分析还允许鉴定与先前与特应性相关的LGG丰度负相关的分类群，以及那些正相关的分类群，这些分类群可以证明作为替代益生菌物种的研究的有用的替代靶标。从这些发现中，我们假设LGG补充剂对随后过敏性疾病发展的保护作用的关键机制是通过促进稳定，均匀和功能冗余的婴儿胃肠道群落。
Colonization of the infant gut by microorganisms over the first year of life is crucial for development of a balanced immune response. Early alterations in the gastrointestinal microbiota of neonates has been linked with subsequent development of asthma and atopy in older children. Here we describe high-resolution culture-independent analysis of stool samples from 6-month old infants fed daily supplements of Lactobacillus casei subsp. Rhamnosus (LGG) or placebo in a double-blind, randomized Trial of Infant Probiotic Supplementation (TIPS). Bacterial community composition was examined using a high-density microarray, the 16S rRNA PhyloChip, and the microbial assemblages of infants with either high or low LGG abundance were compared. Communities with high abundance of LGG exhibited promotion of phylogenetically clustered taxa including a number of other known probiotic species, and were significantly more even in their distribution of community members. Ecologically, these aspects are characteristic of communities that are more resistant to perturbation and outgrowth of pathogens. PhyloChip analysis also permitted identification of taxa negatively correlated with LGG abundance that have previously been associated with atopy, as well as those positively correlated that may prove useful alternative targets for investigation as alternative probiotic species. From these findings we hypothesize that a key mechanism for the protective effect of LGG supplementation on subsequent development of allergic disease is through promotion of a stable, even, and functionally redundant infant gastrointestinal community.
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DOI：
10.1073/pnas.0608255104
发表时间：
2007-01-02
影响因子：
11.1
作者：
Brodie, Eoin L.;DeSantis, Todd Z.;Andersen, Gary L.
通讯作者：
Andersen, Gary L.
影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
影响因子：
9.4
作者：
Flanagan, J. L.;Brodie, E. L.;Bristow, J.
通讯作者：
Bristow, J.
影响因子：
56.9
作者：
Gill, Steven R.;Pop, Mihai;Nelson, Karen E.
通讯作者：
Nelson, Karen E.
影响因子：
3.7
作者：
Andersson, Anders F.;Lindberg, Mathilda;Jakobsson, Hedvig;Backhed, Fredrik;Nyren, Pal;Engstrand, Lars
通讯作者：
Engstrand, Lars