Rice protein phosphatase 1 regulatory subunits OsINH2 and OsINH3 participate actively in growth and adaptive responses under abscisic acid.
Rice protein phosphatase 1 regulatory subunits OsINH2 and OsINH3 participate actively in growth and adaptive responses under abscisic acid.
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水稻蛋白磷酸酶1调节亚基OsINH2和OsINH3积极参与脱落酸下的生长和适应性反应
DOI：
10.3389/fpls.2022.990575
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发表时间：
2022
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
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作者：
水稻 (Oryza sativa L.) 是一种世界性的主粮作物，受到各种环境压力的影响，最终导致产量下降。然而，多样化的生理和分子反应使其能够应对不利因素。它包括众多信号传导的整合，其中蛋白磷酸酶 1 (PP1) 发挥着关键作用。对 PP1 的研究主要局限于众多细胞进程中的 PP1 催化亚基。因此，我们重点研究了拟南芥中 PP1 调节亚基（PP1r）、OsINH2 和 OsINH3（AtINH2 和 AtINH3 的同源物）在水稻生长和逆境适应中的作用。我们的观察表明，这些是普遍表达的调节亚基，与其相对伙伴 1 型蛋白磷酸酶 (OsTOPP) 相互作用并共定位，但不能改变其亚细胞定位。 OsINH2和OsINH3的突变降低了花粉活力，从而影响了水稻的育性。它们可能通过与渗透压/ABA 激活蛋白激酶 (OsSAPK) 相互作用来参与脱落酸 (ABA) 介导的种子萌发抑制。同时，它们通过脯氨酸生物合成积极参与渗透压调节，通过过氧化物酶（POD）解毒活性氧（ROS），减少丙二醛形成（MDA），并调节应激反应基因。此外，它们的相互作用表明它们可能共同或通过共同调节来介导细胞过程。然而，两种不同 PP1r 的特殊行为还需要探索。简而言之，这项研究揭示了 OsINH2 和 OsINH3 在水稻生殖生长中的参与以及逆境下的适应性策略。因此，它们与 ABA 成分的遗传相互作用以及渗透调节和 ROS 调节的深层机制将解释它们在复杂信号传导中的作用。该研究为引进抗逆作物提供了基础。
Rice (Oryza sativa L.), a worldwide staple food crop, is affected by various environmental stressors that ultimately reduce yield. However, diversified physiological and molecular responses enable it to cope with adverse factors. It includes the integration of numerous signaling in which protein phosphatase 1 (PP1) plays a pivotal role. Research on PP1 has been mostly limited to the PP1 catalytic subunit in numerous cellular progressions. Therefore, we focused on the role of PP1 regulatory subunits (PP1r), OsINH2 and OsINH3, homologs of AtINH2 and AtINH3 in Arabidopsis, in rice growth and stress adaptations. Our observations revealed that these are ubiquitously expressed regulatory subunits that interacted and colocalized with their counter partners, type 1 protein phosphatase (OsTOPPs) but could not change their subcellular localization. The mutation in OsINH2 and OsINH3 reduced pollen viability, thereby affected rice fertility. They were involved in abscisic acid (ABA)-mediated inhibition of seed germination, perhaps by interacting with osmotic stress/ABA-activated protein kinases (OsSAPKs). Meanwhile, they positively participated in osmotic adjustment by proline biosynthesis, detoxifying reactive oxygen species (ROS) through peroxidases (POD), reducing malondialdehyde formation (MDA), and regulating stress-responsive genes. Moreover, their co-interaction proposed they might mediate cellular processes together or by co-regulation; however, the special behavior of two different PP1r is needed to explore. In a nutshell, this research enlightened the involvement of OsINH2 and OsINH3 in the reproductive growth of rice and adaptive strategies under stress. Hence, their genetic interaction with ABA components and deep mechanisms underlying osmotic regulation and ROS adjustment would explain their role in complex signaling. This research offers the basis for introducing stress-resistant crops.
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影响因子：
7.3
作者：
Chen, Qing-Bin;Wang, Wen-Jing;Zhang, Yue;Zhan, Qi-Di;Liu, Kang;Botella, Jose Ramon;Bai, Ling;Song, Chun-Peng
通讯作者：
Song, Chun-Peng
影响因子：
16.6
作者：
Boevink PC;Wang X;McLellan H;He Q;Naqvi S;Armstrong MR;Zhang W;Hein I;Gilroy EM;Tian Z;Birch PRJ
通讯作者：
Birch PRJ
影响因子：
5.3
作者：
Diedhiou, Calliste J.;Popova, Olga V.;Golldack, Dortje
通讯作者：
Golldack, Dortje
影响因子：
6.5
作者：
Djanaguiraman, M.;Prasad, P. V. V.;Seppanen, M.
通讯作者：
Seppanen, M.
影响因子：
1.5
作者：
Ding, W.;Song, L.;Bi, Y.
通讯作者：
Bi, Y.