Subgingival Microbiome in Pregnancy and a Potential Relationship to Early Term Birth.
Subgingival Microbiome in Pregnancy and a Potential Relationship to Early Term Birth.
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DOI：
10.3389/fcimb.2022.873683
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发表时间：
2022
影响因子：
5.7
通讯作者：
中科院分区：
文献类型：
--
作者：
妊娠期牙周病被认为是不良分娩结果的危险因素。牙周病有微生物病因，然而，目前对妊娠期龈下微生物组的了解尚不清楚。表征妊娠早期和晚期龈下微生物组的结构和多样性，并探讨黑人孕妇龈下微生物组与早产之间的关系。这项纵向描述性研究使用 16S rRNA 测序来分析 59 名黑人女性的龈下微生物组，并使用 α 和 β 多样性指标描述微生物生态学。我们还根据出生结果时的孕龄（自发性早产、自发性早产、足月）比较了整个妊娠早期（8-14 周）和晚期（24-30 周）的微生物组特征。在这个黑人孕妇样本中，龈下微生物群中代表的前二十个细菌分类群包括代表导致牙周病的生物膜进展各个阶段的谱系，包括已知的牙周病原体牙龈卟啉单胞菌和连翘坦纳菌。龈下微生物组中反映的与牙周病相关的其他微生物包括几种普氏菌属和弯曲杆菌属。 α或β多样性的测量并不能根据妊娠早期/晚期或足月/自发早产来区分女性的龈下微生物组；然而，早产儿自然分娩的妇女和足月分娩的妇女在妊娠晚期的α多样性存在差异。根据妊娠早期/晚期以及足月/自发早产，一些分类群也被确定为丰度差异。尽管龈下微生物组的组成转向与牙周病相关的复合体，但微生物组的多样性在整个怀孕期间保持稳定。一些分类单元被确定与自发性早产有关。其中两个是有希望进一步研究的目标。妊娠早期口腔共生奇异果的消耗和妊娠晚期产黑色素普雷沃菌的水平升高均与自发性早产有关。
Periodontal disease in pregnancy is considered a risk factor for adverse birth outcomes. Periodontal disease has a microbial etiology, however, the current state of knowledge about the subgingival microbiome in pregnancy is not well understood. To characterize the structure and diversity of the subgingival microbiome in early and late pregnancy and explore relationships between the subgingival microbiome and preterm birth among pregnant Black women. This longitudinal descriptive study used 16S rRNA sequencing to profile the subgingival microbiome of 59 Black women and describe microbial ecology using alpha and beta diversity metrics. We also compared microbiome features across early (8-14 weeks) and late (24-30 weeks) gestation overall and according to gestational age at birth outcomes (spontaneous preterm, spontaneous early term, full term). In this sample of Black pregnant women, the top twenty bacterial taxa represented in the subgingival microbiome included a spectrum representative of various stages of biofilm progression leading to periodontal disease, including known periopathogens Porphyromonas gingivalis and Tannerella forsythia. Other organisms associated with periodontal disease reflected in the subgingival microbiome included several Prevotella spp., and Campylobacter spp. Measures of alpha or beta diversity did not distinguish the subgingival microbiome of women according to early/late gestation or full term/spontaneous preterm birth; however, alpha diversity differences in late pregnancy between women who spontaneously delivered early term and women who delivered full term were identified. Several taxa were also identified as being differentially abundant according to early/late gestation, and full term/spontaneous early term births. Although the composition of the subgingival microbiome is shifted toward complexes associated with periodontal disease, the diversity of the microbiome remains stable throughout pregnancy. Several taxa were identified as being associated with spontaneous early term birth. Two, in particular, are promising targets of further investigation. Depletion of the oral commensal Lautropia mirabilis in early pregnancy and elevated levels of Prevotella melaninogenica in late pregnancy were both associated with spontaneous early term birth.
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影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
6.7
作者：
Darby, IB;Hedge, PJ;Kinane, DF
通讯作者：
Kinane, DF
DOI：
10.14219/jada.archive.2010.0228
发表时间：
2010-05-01
影响因子：
3.9
作者：
Boggess, Kim A.;Urlaub, Diana M.;Lorenz, Carol
通讯作者：
Lorenz, Carol
影响因子：
4.3
作者：
COHEN, DW;FRIEDMAN, L;KYLE, GC
通讯作者：
KYLE, GC
影响因子：
4.3
作者：
Adriaens, Laurence A.;Alessandri, Regina;Persson, G. Rutger
通讯作者：
Persson, G. Rutger