Coherent soft X-ray diffraction imaging of coliphage PR772 at the Linac coherent light source.
Coherent soft X-ray diffraction imaging of coliphage PR772 at the Linac coherent light source.
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DOI：
10.1038/sdata.2017.79
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发表时间：
2017-06-27
期刊：
影响因子：
9.8
通讯作者：
Xavier PL
中科院分区：
文献类型：
--
作者：
Reddy HKN;Yoon CH;Aquila A;Awel S;Ayyer K;Barty A;Berntsen P;Bielecki J;Bobkov S;Bucher M;Carini GA;Carron S;Chapman H;Daurer B;DeMirci H;Ekeberg T;Fromme P;Hajdu J;Hanke MF;Hart P;Hogue BG;Hosseinizadeh A;Kim Y;Kirian RA;Kurta RP;Larsson DSD;Duane Loh N;Maia FRNC;Mancuso AP;Mühlig K;Munke A;Nam D;Nettelblad C;Ourmazd A;Rose M;Schwander P;Seibert M;Sellberg JA;Song C;Spence JCH;Svenda M;Van der Schot G;Vartanyants IA;Williams GJ;Xavier PL
X射线自由电子激光的单粒子衍射提供了在不需要结晶的情况下确定分子结构的可能性。为了进一步发展这项技术，我们提供了一组在原子分子光学(AMO)光束线上收集的、在直线加速器相干光源的LAMP仪器中使用pnCCD探测器的大肠杆菌PR772病毒的相干软X射线衍射图像。PR772的直径在65-70 nm之间，远小于以前报道的直径约600 nm的迷你病毒。这反映了基于XFEL的单粒子成像朝着单分子成像制度的持续进展。与之前的实验相比，该数据集包含的单粒子命中率明显更高，从而使改进的统计分析、重建算法和定量度量得以开发，以确定分辨率和自一致性。
Single-particle diffraction from X-ray Free Electron Lasers offers the potential for molecular structure determination without the need for crystallization. In an effort to further develop the technique, we present a dataset of coherent soft X-ray diffraction images of Coliphage PR772 virus, collected at the Atomic Molecular Optics (AMO) beamline with pnCCD detectors in the LAMP instrument at the Linac Coherent Light Source. The diameter of PR772 ranges from 65–70 nm, which is considerably smaller than the previously reported ~600 nm diameter Mimivirus. This reflects continued progress in XFEL-based single-particle imaging towards the single molecular imaging regime. The data set contains significantly more single particle hits than collected in previous experiments, enabling the development of improved statistical analysis, reconstruction algorithms, and quantitative metrics to determine resolution and self-consistency.
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影响因子：
35
作者：
Emma, P.;Akre, R.;Galayda, J.
通讯作者：
Galayda, J.
影响因子：
64.8
作者：
Seibert, M. Marvin;Ekeberg, Tomas;Maia, Filipe R. N. C.;Svenda, Martin;Andreasson, Jakob;Joensson, Olof;Odic, Dusko;Iwan, Bianca;Rocker, Andrea;Westphal, Daniel;Hantke, Max;DePonte, Daniel P.;Barty, Anton;Schulz, Joachim;Gumprecht, Lars;Coppola, Nicola;Aquila, Andrew;Liang, Mengning;White, Thomas A.;Martin, Andrew;Caleman, Carl;Stern, Stephan;Abergel, Chantal;Seltzer, Virginie;Claverie, Jean-Michel;Bostedt, Christoph;Bozek, John D.;Boutet, Sebastien;Miahnahri, A. Alan;Messerschmidt, Marc;Krzywinski, Jacek;Williams, Garth;Hodgson, Keith O.;Bogan, Michael J.;Hampton, Christina Y.;Sierra, Raymond G.;Starodub, Dmitri;Andersson, Inger;Bajt, Sasa;Barthelmess, Miriam;Spence, John C. H.;Fromme, Petra;Weierstall, Uwe;Kirian, Richard;Hunter, Mark;Doak, R. Bruce;Marchesini, Stefano;Hau-Riege, Stefan P.;Frank, Matthias;Shoeman, Robert L.;Lomb, Lukas;Epp, Sascha W.;Hartmann, Robert;Rolles, Daniel;Rudenko, Artem;Schmidt, Carlo;Foucar, Lutz;Kimmel, Nils;Holl, Peter;Rudek, Benedikt;Erk, Benjamin;Hoemke, Andre;Reich, Christian;Pietschner, Daniel;Weidenspointner, Georg;Strueder, Lothar;Hauser, Guenter;Gorke, Hubert;Ullrich, Joachim;Schlichting, Ilme;Herrmann, Sven;Schaller, Gerhard;Schopper, Florian;Soltau, Heike;Kuehnel, Kai-Uwe;Andritschke, Robert;Schroeter, Claus-Dieter;Krasniqi, Faton;Bott, Mario;Schorb, Sebastian;Rupp, Daniela;Adolph, Marcus;Gorkhover, Tais;Hirsemann, Helmut;Potdevin, Guillaume;Graafsma, Heinz;Nilsson, Bjoern;Chapman, Henry N.;Hajdu, Janos
通讯作者：
Hajdu, Janos
影响因子：
9.8
作者：
Munke A;Andreasson J;Aquila A;Awel S;Ayyer K;Barty A;Bean RJ;Berntsen P;Bielecki J;Boutet S;Bucher M;Chapman HN;Daurer BJ;DeMirci H;Elser V;Fromme P;Hajdu J;Hantke MF;Higashiura A;Hogue BG;Hosseinizadeh A;Kim Y;Kirian RA;Reddy HK;Lan TY;Larsson DS;Liu H;Loh ND;Maia FR;Mancuso AP;Mühlig K;Nakagawa A;Nam D;Nelson G;Nettelblad C;Okamoto K;Ourmazd A;Rose M;van der Schot G;Schwander P;Seibert MM;Sellberg JA;Sierra RG;Song C;Svenda M;Timneanu N;Vartanyants IA;Westphal D;Wiedorn MO;Williams GJ;Xavier PL;Yoon CH;Zook J
通讯作者：
Zook J
影响因子：
8.6
作者：
Ekeberg, Tomas E;Svenda, Martin;Hajdu, Janos
通讯作者：
Hajdu, Janos
影响因子：
64.8
作者：
Neutze, R;Wouts, R;Hajdu, J
通讯作者：
Hajdu, J