Chemotaxonomic Classification Applied to the Identification of Two Closely-Related Citrus TCMs Using UPLC-Q-TOF-MS-Based Metabolomics.
Chemotaxonomic Classification Applied to the Identification of Two Closely-Related Citrus TCMs Using UPLC-Q-TOF-MS-Based Metabolomics.
复制标题
使用基于 UPLC-Q-TOF-MS 的代谢组学将化学分类学分类应用于两种密切相关的柑橘类中药的鉴定
DOI：
10.3390/molecules22101721
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发表时间：
2017-10-13
期刊：
影响因子：
--
通讯作者：
Liu YY
中科院分区：
文献类型：
--
作者：
Zhao SY;Liu ZL;Shu YS;Wang ML;He D;Song ZQ;Zeng HL;Ning ZC;Lu C;Lu AP;Liu YY
本文阐述了建立中药材柑橘近缘种的化学分类策略。应用UPLC-Q-TOF-MS代谢组学技术对柑橘不同品种、不同成熟期的中药材进行化学分类，揭示不同品种、不同成熟期柑橘中药材的代谢机制。代谢组学可以获取对小分子代谢物的全面分析，并可以提供一种强大的方法来建立代谢谱，在基因型和表型之间建立一座桥梁。为了进一步研究四种柑橘类中药中不同的代谢物，采用非靶向代谢物图谱分析作为一种有效的初级和次生代谢物图谱分析技术。结果表明，初级代谢物能够区分物种，而次生代谢物与物种和成熟过程有关。此外，对生物合成途径的分析表明，在柑橘成熟期，黄酮类和黄酮苷的合成受到很大影响。最终，这项工作可能会为不同物种和成熟阶段的柑橘果实的鉴定提供一种可行的策略，并有助于更好地了解它们的不同药用价值。
This manuscript elaborates on the establishment of a chemotaxonomic classification strategy for closely-related Citrus fruits in Traditional Chinese Medicines (TCMs). UPLC-Q-TOF-MS-based metabolomics was applied to depict the variable chemotaxonomic markers and elucidate the metabolic mechanism of Citrus TCMs from different species and at different ripening stages. Metabolomics can capture a comprehensive analysis of small molecule metabolites and can provide a powerful approach to establish metabolic profiling, creating a bridge between genotype and phenotype. To further investigate the different metabolites in four closely-related Citrus TCMs, non-targeted metabolite profiling analysis was employed as an efficient technique to profile the primary and secondary metabolites. The results presented in this manuscript indicate that primary metabolites enable the discrimination of species, whereas secondary metabolites are associated with species and the ripening process. In addition, analysis of the biosynthetic pathway highlighted that the syntheses of flavone and flavone glycosides are deeply affected in Citrus ripening stages. Ultimately, this work might provide a feasible strategy for the authentication of Citrus fruits from different species and ripening stages and facilitate a better understanding of their different medicinal uses.
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影响因子：
6.2
作者：
Kim, Young Mi;Lee, Joongku;Lee, Choong Hwan
通讯作者：
Lee, Choong Hwan
影响因子：
6.1
作者：
Dai, Weidong;Qi, Dandan;Lin, Zhi
通讯作者：
Lin, Zhi
柑橘类水果是活性天然代谢物的宝库，可能为人类健康带来益处
影响因子：
--
作者：
Lv X;Zhao S;Ning Z;Zeng H;Shu Y;Tao O;Xiao C;Lu C;Liu Y
通讯作者：
Liu Y
影响因子：
4.6
作者：
Jia HM;Li Q;Zhou C;Yu M;Yang Y;Zhang HW;Ding G;Shang H;Zou ZM
通讯作者：
Zou ZM
影响因子：
4.1
作者：
Guo, Sheng;Duan, Jin-ao;Shang, Erxin
通讯作者：
Shang, Erxin