Cloning and characterization of the HLIP gene encoding high light-inducible protein from Porphyra yezoensis

Cloning and characterization of the HLIP gene encoding high light-inducible protein from Porphyra yezoensis
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条斑紫菜强光诱导蛋白HLIP基因的克隆与鉴定

DOI:
10.1007/s10811-011-9686-z
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发表时间:
2012-08
影响因子:
3.3
通讯作者:
Li Wang
Li Wang
中科院分区:
生物学3区
文献类型:
--
作者:
Fanna Kong;Yunxiang Mao;Hui Yang;Li Wang

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强光诱导蛋白在光合作用生物响应强光胁迫时起着至关重要的作用。我们克隆并鉴定了条斑紫菜的HLIP基因,命名为PyHLIP。序列分析表明,pYHLIP的开放阅读框全长387hp,编码128个氨基酸的多肽,含有一个叶绿体转运肽和一个明显的跨膜α-螺旋区域。PYHLIP基因组DNA中不存在内含子。PYHLIP的启动子不含TATA,ATG上游存在启动子元件(INR)。基因组组织分析表明,条斑病菌核基因组中含有两个拷贝的pYHLIP基因。PyHLIP的表达可被不同的胁迫条件诱导,尤其对强光敏感。
High light-inducible proteins are critical for photosynthetic organisms when responding to high light stress. We cloned and characterized the HLIP gene from Porphyra yezoensis, which was designated as PyHLIP. Sequence analysis revealed that the open reading frame of PyHLIP was 387 bp in length that encoded a 128 amino acid-polypeptide containing a plastid transit peptide and an obvious membrane-spanning α-helix region. No intron existed in the genomic DNA of PyHLIP. The putative promoter of PyHLIP was TATA-less, and the initiator element (Inr) existed upstream of ATG. Analysis of genomic organization indicated there were two copies of PyHLIP gene in the P. yezoensis nuclear genome. The expression of PyHLIP can be induced by various stress conditions and is especially sensitive to high light.
DOI: 10.1016/b978-0-12-809633-8.20106-4
发表时间: 2020-02
期刊: --
影响因子: --
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