Tobacco smoke exposure induces nicotine dependence in rats.
Tobacco smoke exposure induces nicotine dependence in rats.
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DOI：
10.1007/s00213-009-1716-z
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发表时间：
2010-01
影响因子：
3.4
通讯作者：
Bruijnzeel, Adrie W.
中科院分区：
文献类型：
--
作者：
Small, Elysia;Shah, Hina P.;Davenport, Jake J.;Geier, Jacqueline E.;Yavarovich, Kate R.;Yamada, Hidetaka;Sabarinath, Sreedharan N.;Derendorf, Hartmut;Pauly, James R.;Gold, Mark S.;Bruijnzeel, Adrie W.
烟草烟雾含有尼古丁和许多其他化合物，这些化合物对大脑奖励系统有协同作用。因此，需要动物模型，允许调查慢性暴露于烟草烟雾成分的全谱。这些研究的目的是调查暴露于烟草烟雾是否会导致大鼠尼古丁依赖。颅内自我刺激程序被用来评估尼古丁戒断的负面情感方面。从尼古丁戒断体征清单中记录躯体体征。进行尼古丁自我给药会议，以调查烟草烟雾暴露是否影响自我给药尼古丁的动机。尼古丁受体放射自显影术用于研究烟草烟雾暴露是否影响中枢α7烟碱乙酰胆碱受体（nAChR）和非α7 nAChR（主要是α4β2 nAChR）水平。nAChR拮抗剂美加明剂量依赖性地升高暴露于烟草烟雾的大鼠的脑奖励阈值，并且不影响未处理的对照大鼠的脑奖励阈值。此外，美加明诱导更多的躯体戒断症状在烟雾暴露的大鼠比对照组大鼠。尼古丁自我给药在最后一次烟草烟雾暴露后1天减少，5天后恢复到对照水平。烟草烟雾暴露可使海马CA 2/3区和皮层α7 nAChR密度增加，齿状回非α7 nAChR密度增加。烟草烟雾暴露导致尼古丁依赖，如沉淀的情感和躯体戒断症状所示，并诱导海马中nAChR的上调。
Tobacco smoke contains nicotine and many other compounds that act in concert on the brain reward system. Therefore, animal models are needed that allow the investigation of chronic exposure to the full spectrum of tobacco smoke constituents. The aim of these studies was to investigate if exposure to tobacco smoke leads to nicotine dependence in rats. The intracranial self-stimulation procedure was used to assess the negative affective aspects of nicotine withdrawal. Somatic signs were recorded from a checklist of nicotine abstinence signs. Nicotine self-administration sessions were conducted to investigate if tobacco smoke exposure affects the motivation to self-administer nicotine. Nicotinic receptor autoradiography was used to investigate if exposure to tobacco smoke affects central α7 nicotinic acetylcholine receptor (nAChR) and non-α7 nAChR levels (primarily α4β2 nAChRs). The nAChR antagonist mecamylamine dose-dependently elevated the brain reward thresholds of the rats exposed to tobacco smoke and did not affect the brain reward thresholds of the untreated control rats. Furthermore, mecamylamine induced more somatic withdrawal signs in the smoke exposed rats than in the control rats. Nicotine self-administration was decreased 1 day after the last tobacco smoke exposure sessions and was returned to control levels 5 days later. Tobacco smoke exposure increased the α7 nAChR density in the CA2/3 area and the stratum oriens and increased the non-α7 nAChR density in the dentate gyrus. Tobacco smoke exposure leads to nicotine dependence as indicated by precipitated affective and somatic withdrawal signs and induces an upregulation of nAChRs in the hippocampus.
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影响因子：
3.4
作者：
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通讯作者：
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