Livestock-associated methicillin-resistant Staphylococcus aureus sequence type 398 in humans, Canada.
Livestock-associated methicillin-resistant Staphylococcus aureus sequence type 398 in humans, Canada.
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DOI：
10.3201/eid1604.091435
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发表时间：
2010-04
影响因子：
11.8
通讯作者：
Mulvey MR
中科院分区：
文献类型：
--
作者：
Golding GR;Bryden L;Levett PN;McDonald RR;Wong A;Wylie J;Graham MR;Tyler S;Van Domselaar G;Simor AE;Gravel D;Mulvey MR
最近出现的由这种菌株引起的感染是公共卫生问题。在加拿大，猪和养猪户中与家畜相关的耐甲氧西林金黄色葡萄球菌（MRSA）序列类型398的定殖率很高，但一般人群的患病率尚不清楚。在这项研究中，从来自加拿大马尼托巴和萨斯喀彻温省的3，687株受试MRSA分离株中鉴定出5株LA-MRSA分离株，其中4株来自皮肤和软组织感染。进一步的分子特征确定，这些菌株都含有葡萄球菌盒式染色体（SCC）的mecV，是阴性的Panton-Valentine杀白细胞素，并密切相关的限制性内切酶Cfr 91的宏观限制性内切酶分析。分离物SCCmec区域的完整DNA序列显示了SCCmecV的一种新亚型，其具有成簇的规则间隔的短回文重复序列和相关基因。虽然家畜相关MRSA在加拿大的一般人群中的流行率似乎较低，但最近出现的由该菌株引起的感染是公共卫生问题。
Recent emergence of infections resulting from this strain is of public health concern. Rates of colonization with livestock-associated methicillin-resistant Staphylococcus aureus (MRSA) sequence type 398 have been high for pigs and pig farmers in Canada, but prevalence rates for the general human population are unknown. In this study, 5 LA-MRSA isolates, 4 of which were obtained from skin and soft tissue infections, were identified from 3,687 tested MRSA isolates from persons in Manitoba and Saskatchewan, Canada. Further molecular characterization determined that these isolates all contained staphylococcal cassette chromosome (SCC) mecV, were negative for Panton-Valentine leukocidin, and were closely related by macrorestriction analysis with the restriction enzyme Cfr91. The complete DNA sequence of the SCCmec region from the isolate showed a novel subtype of SCCmecV harboring clustered regularly interspaced short palindromic repeats and associated genes. Although prevalence of livestock-associated MRSA seems to be low for the general population in Canada, recent emergence of infections resulting from this strain is of public health concern.
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DOI：
10.1126/science.1165771
发表时间：
2008-12-19
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Marraffini LA;Sontheimer EJ
通讯作者：
Sontheimer EJ
影响因子：
14.9
作者：
Grissa, Ibtissem;Vergnaud, Gilles;Pourcel, Christine
通讯作者：
Pourcel, Christine
影响因子：
11.8
作者：
van Loo I;Huijsdens X;Tiemersma E;de Neeling A;van de Sande-Bruinsma N;Beaujean D;Voss A;Kluytmans J
通讯作者：
Kluytmans J
影响因子：
3.3
作者：
Khanna, T.;Friendship, R.;Weese, J. S.
通讯作者：
Weese, J. S.
影响因子：
11.8
作者：
van Rijen, Miranda Maria Lucia;Van Keulen, Peter H.;Kluytmans, Jan A.
通讯作者：
Kluytmans, Jan A.