The kinetics of urinary fumonisin B1 excretion in humans consuming maize-based diets.
The kinetics of urinary fumonisin B1 excretion in humans consuming maize-based diets.
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DOI：
10.1002/mnfr.201200166
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发表时间：
2012-09
影响因子：
5.2
通讯作者：
Ashley-Koch, Allison E.
中科院分区：
文献类型：
--
作者：
Riley, Ronald T.;Torres, Olga;Showker, Jency L.;Zitomer, Nicholas C.;Matute, Jorge;Voss, Kenneth A.;Gelineau-van Waes, Janee;Maddox, Joyce R.;Gregory, Simon G.;Ashley-Koch, Allison E.
伏马菌素（FB）是玉米中发现的真菌毒素。本研究的目的是：1）确定人体中FB 1、FB 2和FB 3摄入量与尿液排泄量之间的关系，2）验证用于国际运输的C18-SPE柱上分离尿液FB的方法，3）使用危地马拉样本检测该方法。志愿者（n=10）消耗206克/天的玉米粉圆饼和饼干制备的湿润粉糊粉和含有玉米粉的产品。志愿者估计其每日尿量，并分析样本中的FB 1、FB 2和FB 3以及水解FB 1。在尿液中仅检测到FB 1，表明FB 2和FB 3的吸收较低。排泄变化很大，开始进食后很快达到峰值，停止进食后迅速下降。在消费结束后的五天内，在尿液中未检测到FB 1。在一项有8名志愿者参与的研究中，平均尿FB 1总量为摄入量的0.5%。在危地马拉采集的样本中，61%（107/177）检测到FB 1。结果支持在基于人群的研究中使用尿FB 1评估持续暴露。然而，由于个体间的变异性和清除的快速性，将个体受试者尿液中的FB 1浓度与FB的饮食摄入量相关联将是复杂的。
Fumonisins (FB) are mycotoxins found in maize. The purpose of this study was to 1) determine the relationship between FB1, FB2 and FB3 intake and urinary excretion in humans, 2) validate a method to isolate urinary FB on C18-SPE cartridges for international shipment, and 3) test the method using samples from Guatemala. Volunteers (n=10) consumed 206 grams/day of tortillas and biscuits prepared from masa flour and a product containing maize flour. Volunteers estimated their daily urine output and samples were analyzed for FB1, FB2 and FB3 and hydrolyzed FB1. Only FB1 was detected in urine suggesting lower absorption of FB2 and FB3. Excretion was highly variable peaking soon after consumption began and decreasing rapidly after consumption stopped. Within five days after consumption ended FB1 was not detected in urine. In a study with eight volunteers, the average total urinary FB1 was 0.5% of the intake. FB1 was detected in 61% (107/177) of the samples collected in Guatemala. The results support the use of urinary FB1 to assess ongoing exposure in population based studies. However, relating the FB1 concentration in urine to dietary intake of FB by individual subjects will be complicated due to inter-individual variability and the rapidity of clearance.
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影响因子：
3.8
作者：
van der Westhuizen, Liana;Shephard, Gordon S.;Gong, Yun Yun
通讯作者：
Gong, Yun Yun
影响因子：
4.2
作者：
Palencia, E;Torres, O;Riley, RT
通讯作者：
Riley, RT
影响因子：
4.3
作者：
Zitomer, Nicholas C.;Glenn, Anthony E.;Riley, Ronald T.
通讯作者：
Riley, Ronald T.
DOI：
10.1007/978-1-61779-102-4_15
发表时间：
2011-01-01
期刊：
MICROBIAL TOXINS: METHODS AND PROTOCOLS
影响因子：
--
作者：
Zitomer, Nicholas C.;Riley, Ronald T.
通讯作者：
Riley, Ronald T.
影响因子：
3.8
作者：
Gong, Yun Yun;Torres-Sanchez, Luisa;Wild, Christopher P.
通讯作者：
Wild, Christopher P.