Functional electrical stimulation helps replenish progenitor cells in the injured spinal cord of adult rats.
Functional electrical stimulation helps replenish progenitor cells in the injured spinal cord of adult rats.
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DOI：
10.1016/j.expneurol.2009.12.029
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发表时间：
2010-04
影响因子：
5.3
通讯作者：
McDonald, John W.
中科院分区：
文献类型：
--
作者：
Becker, Daniel;Gary, Devin S.;Rosenzweig, Ephron S.;Grill, Warren M.;McDonald, John W.
功能性电刺激（FES）可以恢复控制和抵消神经损伤后肌肉萎缩。然而，FES尚未被认为是增强CNS再生的方法。这篇论文证明了FES显著地增强了慢性脊髓损伤（SCI）大鼠脊髓中祖细胞的生成。在12只大鼠的胸8/9水平上进行完全SCI。三周后，将刺激后肢运动的FES装置植入这些大鼠中。12个相同的受伤的大鼠接受非活性FES植入物。还检查了未受伤大鼠的额外对照组。FES植入后10天，用溴脱氧尿苷（BrdU）标记分裂细胞。在完成BrdU给药后立即处死“细胞出生”亚组（每组一半动物），7天后处死“细胞存活”亚组。在受伤的“细胞出生”亚组中，FES诱导腰椎脊髓中细胞出生增加82- 86%。在受损的“细胞存活”亚组中，腰椎新生细胞计数持续增加。FES使表达巢蛋白和其他提示三能祖细胞的标记物的新生细胞的比例增加了一倍。在未受伤的大鼠中，FES对细胞出生/存活没有影响。这份报告表明，控制电激活中枢神经系统可能会增强神经损伤后的自发再生。
Functional electrical stimulation (FES) can restore control and offset atrophy to muscles after neurological injury. However, FES has not been considered as a method for enhancing CNS regeneration. This paper demonstrates that FES dramatically enhanced progenitor cell birth in the spinal cord of rats with a chronic spinal cord injury (SCI). A complete SCI at thoracic level 8/9 was performed on 12 rats. Three weeks later, a FES device to stimulate hindlimb movement was implanted into these rats. Twelve identically-injured rats received inactive FES implants. An additional control group of uninjured rats were also examined. Ten days after FES implantation, dividing cells were marked with bromodeoxyuridine (BrdU). The ‘cell birth’ subgroup (half the animals in each group) was sacrificed immediately after completion of BrdU administration, and the ‘cell survival’ subgroup was sacrificed 7 days later. In the injured ‘cell birth’ subgroup, FES induced an 82-86 % increase in cell birth in the lumbar spinal cord. In the injured ‘cell survival’ subgroup, the increased lumbar newborn cell counts persisted. FES doubled the proportion of the newly-born cells which expressed nestin and other markers suggestive of tripotential progenitors. In uninjured rats, FES had no effect on cell birth/survival. This report suggests that controlled electrical activation of the CNS may enhance spontaneous regeneration after neurological injuries.
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影响因子：
2.5
作者：
BJUGN, R;GUNDERSEN, HJG
通讯作者：
GUNDERSEN, HJG
影响因子：
3.5
作者：
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影响因子：
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作者：
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5.3
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影响因子：
4.4
作者：
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通讯作者：
Lubetzki, C