Cell separation on antigen-coated columns. Elimination of high rate antibody-forming cells and immunological memory cells.
Cell separation on antigen-coated columns. Elimination of high rate antibody-forming cells and immunological memory cells.
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细胞分离抗原涂层柱。消除高速抗体形成细胞和免疫记忆细胞。
DOI：
10.1084/jem.129.1.23
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发表时间：
1969-01-01
影响因子：
15.3
通讯作者：
ANDERSSON, B
中科院分区：
文献类型：
--
作者：
WIGZELL, H;ANDERSSON, B
用抗原蛋白分子包被的玻璃和塑料珠柱用作含有相关特异性免疫细胞的细胞群的免疫过滤器。定期注意到这些免疫细胞从通过的细胞悬浮液中的选择性消除，并且在一些实验中，其接近完全消除过滤的细胞群的特异性免疫反应性。抗原柱对免疫细胞的这种特异性保留可以通过过滤期间培养基中游离抗原分子的存在而选择性地阻断。所获得的结果支持了细胞相关抗原特异性受体存在于免疫细胞外表面的概念，其显示与细胞的潜在产物体液抗体相同的抗原结合特异性。使用本珠柱系统，获得的结果表明该受体是免疫细胞的活性产物，而不是任何被动吸附的亲细胞抗体。抗原珠柱可以很好地构成用于在体外产生特异性剥夺免疫反应性的细胞群体的工具，并且允许详细分析免疫细胞的细胞相关抗体的特征。
Glass and plastic bead columns coated with antigenic protein molecules were used as an immunological filter for cell populations containing immune cells of relevant specificity. A selective elimination of these immune cells from the passing cell suspension was regularly noted and it approached, in some experiments, complete abolition of the specific immune reactivity of the filtered cell population. This specific retention of immune cells by antigenic columns could be selectively blocked by the presence of free antigen molecules in the medium during filtration. The results obtained support the concept of a cell-associated antigen-specific receptor being present on the outer surface of immune cells, displaying the same antigen-binding specificity as the potential product of the cell, the humoral antibody. Using the present bead column system, results were obtained indicating that this receptor was an active product of the immune cells and not any passively adsorbed, cytophilic antibody. Antigenic bead columns may very well constitute a tool for the production in vitro of cell populations being specifically deprived of immune reactivity and allow detailed analysis of the characteristics of the cell-associated antibody of immune cells.
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影响因子：
64.8
作者：
HUNTER, WM;GREENWOOD, FC
通讯作者：
GREENWOOD, FC
影响因子：
15.3
作者：
CELADA, F
通讯作者：
CELADA, F
影响因子：
15.3
作者：
GELL, PGH;SELL, S
通讯作者：
SELL, S
影响因子：
2.9
作者：
EISEN, HN;SISKIND, GW
通讯作者：
SISKIND, GW
影响因子：
15.3
作者：
WIGZELL, H
通讯作者：
WIGZELL, H