Rigor and reproducibility: status and challenges for single vesicle analysis.
Rigor and reproducibility: status and challenges for single vesicle analysis.
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DOI：
10.20517/evcna.2022.28
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Welsh, Joshua A.
中科院分区：
文献类型：
--
作者：
Nolan, John P.;Chiu, Daniel T.;Welsh, Joshua A.
本报告总结了2021年12月在美国圣地亚哥举行的SELECTBIO EV研究和单EV分析的严谨性和再现性研讨会的演讲和活动。会议的动机是认识到细胞外囊泡（EV）领域的进展受到严格和可重复EV测量工具的限制。这些工具对于电动汽车从研究实验室的好奇心发展到提高我们理解，诊断，治疗和预防疾病的能力是绝对必要的。该计划重点关注最近MISEV 2018和MIFlowCyt-EV出版物中列出的EV测量和表征指南，其在常规实践中的实施，以及随着新EV测量技术的引入而不断发展。研讨会的结论是，需要更多的努力集中在分析前的问题和隔离方法的基准，以加强合作和推进更有效的生物标志物。
This report summarises the presentations and activities of the SELECTBIO Workshop on Rigor and Reproducibility in EV Research and Single EV Analysis held in San Diego, USA, in December 2021. The motivation for the session was the recognition that progress in the extracellular vesicle (EV) field is limited by the availability of rigorous and reproducible EV measurement tools. These tools are absolutely required for EVs to evolve from a research lab curiosity to something that will improve our ability to understand, diagnose, treat, and prevent disease. The program focused on guidelines for EV measurement and characterization as laid out in the recent MISEV2018 and MIFlowCyt-EV publications, their implementation in routine practice, and their continued evolution as new EV measurement technologies are introduced. The conclusion of the workshop was that more effort focused on pre-analytical issues and benchmarking of isolation methods is needed to strengthen collaborations and advance more effective biomarkers.
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工程表达良好的V2免疫焦源，利用HIV-1包膜糖蛋白膜膜三聚体用于异源促进疫苗方案。
影响因子：
6.7
作者：
Crooks ET;Almanza F;D'Addabbo A;Duggan E;Zhang J;Wagh K;Mou H;Allen JD;Thomas A;Osawa K;Korber BT;Tsybovsky Y;Cale E;Nolan J;Crispin M;Verkoczy LK;Binley JM
通讯作者：
Binley JM
影响因子：
3.7
作者：
Lee, Jamie A.;Spidlen, Josef;Boyce, Keith;Cai, Jennifer;Crosbie, Nicholas;Dalphin, Mark;Furlong, Jeff;Gasparetto, Maura.;Goldberg, Michael;Goralczyk, Elizabeth M.;Hyun, Bill;Jansen, Kirstin;Kollmann, Tobias;Kong, Megan;Leif, Robert;McWeeney, Shannon;Moloshok, Thomas D.;Moore, Wayne;Nolan, Garry;Nolan, John;Nikolich-Zugich, Janko;Parrish, David;Purcell, Barclay;Qian, Yu;Selvaraj, Biruntha;Smith, Clayton;Tchuvatkina, Olga;Wertheimer, Anne;WilkinSon, Peter;Wilson, Christopher;Wood, James;Zigon, Robert;Scheuermann, Richard H.;Brinkman, Ryan R.
通讯作者：
Brinkman, Ryan R.
DOI：
10.1111/jth.15540
发表时间：
2022-01
期刊：
Journal of thrombosis and haemostasis : JTH
影响因子：
--
作者：
van der Pol E;Welsh JA;Nieuwland R
通讯作者：
Nieuwland R
影响因子：
7.4
作者：
NGUYEN, DC;KELLER, RA;MARTIN, JC
通讯作者：
MARTIN, JC
影响因子：
5.6
作者：
Shpigelman J;Lao FS;Yao S;Li C;Saito T;Sato-Kaneko F;Nolan JP;Shukla NM;Pu M;Messer K;Cottam HB;Carson DA;Corr M;Hayashi T
通讯作者：
Hayashi T