Benchtop micromolding of polystyrene by soft lithography.
Benchtop micromolding of polystyrene by soft lithography.
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DOI：
10.1039/c1lc20281b
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发表时间：
2011-09-21
期刊：
影响因子：
6.1
通讯作者：
Allbritton NL
中科院分区：
文献类型：
--
作者：
Wang Y;Balowski J;Phillips C;Phillips R;Sims CE;Allbritton NL
聚苯乙烯(PS)是细胞培养耗材实验室的标准材料，通过弹性聚二甲基硅氧烷(PDMS)模具模塑成具有高复制保真度的微结构。该过程是一种简单的台式方法，基于使用现成材料的软光刻。通过这个简单的过程成功复制成型的关键依赖于溶剂的使用，例如伽马-丁内酯，它可以溶解PS而不会使PDMS模具膨胀。将PS溶液加入到PDMS模具中，通过在热板上烘烤模具来完成溶剂的挥发。特征尺寸小到3微米、长宽比大到7的微结构很容易成型。采用热键合技术，将PS中成型的微通道与平面PS基板进行热键合，制备了PS微流控芯片的原型。与由PDMS制成的类似芯片相比，PS微流控芯片对疏水分子(例如罗丹明B)的吸附和吸收要低得多。通过接触角测量表明，经等离子体氧化处理后的PS成型表面具有稳定的表面性能。由于细胞的黏附和增殖，模压、氧化的PS表面仍然是细胞培养的极佳表面。微模成型的PS具有非常适合生物和生物分析需求的特性，因此使其成为PDMS的替代材料，并适合于通过软光刻方法制造芯片上实验室设备。
Polystyrene (PS), a standard material for cell culture consumable labware, was molded into microstructures with high fidelity of replication by an elastomeric polydimethylsiloxane (PDMS) mold. The process was a simple, benchtop method based on soft lithography using readily available materials. The key to successful replica molding by this simple procedure relies on the use of a solvent, for example, gamma-butyrolactone, which dissolves PS without swelling the PDMS mold. PS solution was added to the PDMS mold, and evaporation of solvent was accomplished by baking the mold on a hotplate. Microstructures with feature sizes as small as 3 µm and aspect ratios as large as 7 were readily molded. Prototypes of microfluidic chips made from PS were prepared by thermal bonding of a microchannel molded in PS with a flat PS substrate. The PS microfluidic chip displayed much lower adsorption and absorption of hydrophobic molecules (e.g. rhodamine B) compared to a comparable chip created from PDMS. The molded PS surface exhibited stable surface properties after plasma oxidation as assessed by contact angle measurement. The molded, oxidized PS surface remained an excellent surface for cell culture based on cell adhesion and proliferation. The micromolded PS possessed properties that were ideal for biological and bioanalytical needs, thus making it an alternative material to PDMS and suitable for building lab-on-a-chip devices by soft lithography methods.
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影响因子：
18.3
作者：
Gratton, Stephanie E. A.;Williams, Stuart S.;Napier, Mary E.;Pohlhaus, Patrick D.;Zhou, Zhilian;Wiles, Kenton B.;Maynor, Benjamin W.;Shen, Clifton;Olafsen, Tove;Samulski, Edward T.;Desimone, Joseph M.
通讯作者：
Desimone, Joseph M.
影响因子：
9.9
作者：
Kim, J;Chaudhury, MK;Orbeck, T
通讯作者：
Orbeck, T
影响因子：
2.2
作者：
Fritz, JL;Owen, MJ
通讯作者：
Owen, MJ
影响因子：
7.4
作者：
Barker, SLR;Tarlov, MJ;Locascio, LE
通讯作者：
Locascio, LE
影响因子：
7.4
作者：
Barker, SLR;Ross, D;Locascio, LE
通讯作者：
Locascio, LE