Terpene synthases in cucumber (Cucumis sativus) and their contribution to herbivore-induced volatile terpenoid emission.
Terpene synthases in cucumber (Cucumis sativus) and their contribution to herbivore-induced volatile terpenoid emission.
复制标题
黄瓜中的萜烯合酶（黄瓜）及其对草食动物诱导的挥发性萜类化合物的贡献。
DOI：
10.1111/nph.17814
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发表时间：
2022-01
期刊：
影响因子：
9.4
通讯作者：
Kappers, Iris F.
中科院分区：
文献类型：
--
作者：
He, Jun;Verstappen, Francel;Jiao, Ao;Dicke, Marcel;Bouwmeester, Harro J.;Kappers, Iris F.
关键词：
萜类化合物在植物的香味、传粉者吸引和防御中起着重要作用。在黄瓜（Cucumis sativus）中，它们是吸引食草动物天敌的食草动物诱导的植物挥发性混合物的重要成分。我们注释了黄瓜萜烯合成酶基因（CsTPS）家族，并采用分子和生物化学相结合的方法表征了它们参与对具有不同摄食公会的食草动物的反应。多个CsTPS基因的转录物在食草动物后的叶子中上调，并且由表达的蛋白质产生的产物与食草动物受损的叶子释放的挥发性混合物中记录的萜类化合物相匹配。CsTPS基因启动子活性的空间和时间分析表明，取食细胞内容物的叶螨（二斑叶螨）和蓟马（花蓟马）在侵染开始后数小时内诱导CsTPS 9和CsTPS 19的启动子活性，而取食韧皮部的蚜虫（桃蚜）诱导CsTPS 2启动子活性。我们的研究结果为TPS基因家族参与黄瓜中草食动物诱导的植物挥发物排放的动态和微调提供了详细的见解，并为理解影响植物-草食动物相互作用的分子机制开辟了一条新途径。
Terpenoids play important roles in flavour, pollinator attraction and defence of plants. In cucumber (Cucumis sativus) they are important components of the herbivore‐induced plant volatile blend that attracts natural enemies of herbivores. We annotated the cucumber TERPENE SYNTHASE gene (CsTPS) family and characterized their involvement in the response towards herbivores with different feeding guilds using a combined molecular and biochemical approach. Transcripts of multiple CsTPS genes were upregulated in leaves upon herbivory and the products generated by the expressed proteins match the terpenoids recorded in the volatile blend released by herbivore‐damaged leaves. Spatial and temporal analysis of the promoter activity of CsTPS genes showed that cell content‐feeding spider mites (Tetranychus urticae) and thrips (Frankliniella occidentalis) induced promoter activity of CsTPS9 and CsTPS19 within hours after initiation of infestation, while phloem‐feeding aphids (Myzus persicae) induced CsTPS2 promoter activity. Our findings offer detailed insights into the involvement of the TPS gene family in the dynamics and fine‐tuning of the emission of herbivore‐induced plant volatiles in cucumber, and open a new avenue to understand molecular mechanisms that affect plant–herbivore interactions.
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影响因子：
2.9
作者：
Cao, Rong;Zhang, Yonghui;Mann, Francis M.;Huang, Cancan;Mukkamala, Dushyant;Hudock, Michael P.;Mead, Matthew E.;Prisic, Sladjana;Wang, Ke;Lin, Fu-Yang;Chang, Ting-Kai;Peters, Reuben J.;Odfield, Eric
通讯作者：
Odfield, Eric
影响因子：
3.1
作者：
Aubourg, S;Lecharny, A;Bohlmann, J
通讯作者：
Bohlmann, J
影响因子：
7.4
作者：
Falara, Vasiliki;Akhtar, Tariq A.;Pichersky, Eran
通讯作者：
Pichersky, Eran
影响因子：
2.6
作者：
Gawronska, H;Kielkiewicz, M
通讯作者：
Kielkiewicz, M
影响因子：
7.4
作者：
Ament, K;Kant, MR;Schuurink, RC
通讯作者：
Schuurink, RC