Enhanced production of poly(vinyl alcohol)-degrading enzymes by mixed microbial culture using 1,4-butanediol and designed fermentation strategies
Enhanced production of poly(vinyl alcohol)-degrading enzymes by mixed microbial culture using 1,4-butanediol and designed fermentation strategies
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使用 1,4-丁二醇混合微生物培养和设计的发酵策略提高聚（乙烯醇）降解酶的产量
DOI：
10.1016/j.polymdegradstab.2009.12.015
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发表时间：
2010-04
影响因子：
5.9
通讯作者：
Chen, Jian
中科院分区：
文献类型：
--
作者：
Tang, Bo;Liao, Xianyan;Zhang, Dongxu;Li, Min;Li, Rong;Yan, Kelu;Du, Guocheng;Chen, Jian
目前，聚乙烯醇(PVA)对自然环境造成了严重的污染。为消除PVA污染，研究了PVA降解酶(PVADE)。此前，我们的团队曾在混合微生物培养中检测到PVADE。本研究发现，1，4-丁二醇对PVADE的产生有促进作用。以1，4-丁二醇为碳源，PVADE活力(3.43U/ml−-1)是对照(0.75U/ml−-1)的4.6倍。同时，PVA的平均降解率比对照提高了2.0倍。具体地说，观察到双氧水生长伴随着PVA降解率的增加。基于这一现象，设计了适时添加碳源的两段发酵工艺。通过应用这一策略，获得了较高的PVADE生产率(60.8U/L−1h−1)。此外，通过添加PVA将两段发酵扩展为三段发酵，以提高PVADE的产量。单位生物量的PVADE活性(YPVADE/x)比两级发酵显著提高，最大增量为418U/g−1。
Nowadays, poly(vinyl alcohol) (PVA) has caused serious pollution in the natural environment. To eliminate PVA pollution, PVA-degrading enzymes (PVADE) were studied. Previously our group has detected PVADE in a mixed microbial culture. In this study, it was found that 1,4-butanediol could enhance PVADE production. High PVADE activity (3.43 U ml−1), which was 4.6 folds of the control (0.75 U ml−1), was achieved with 1,4-butanediol as carbon source. Concomitantly, the average PVA-degrading rate improved 2.0 folds compared to the control. Specifically, diauxic growth coupled with increased PVA-degrading rate was observed. Based on this phenomenon, two-stage fermentation by adding another carbon source at a proper time was designed. By applying this strategy, high PVADE productivity (60.8 U l−1h−1) was achieved. Further, the two-stage fermentation was extended to three-stage fermentation by adding PVA to improve PVADE production. The PVADE activity per unit biomass (YPVADE/x) was significantly enhanced over two-stage fermentation and the maximum increment was 418 U g−1.
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影响因子：
4.4
作者：
M. Shimao;Yoshifumi Taniguchi;Shitsuu Shikata;N. Kato;C. Sakazawa
通讯作者：
M. Shimao;Yoshifumi Taniguchi;Shitsuu Shikata;N. Kato;C. Sakazawa
DOI：
10.1016/s0895-3988(08)60013-6
发表时间：
2008-02
期刊：
Biomedical and environmental sciences : BES
影响因子：
--
作者：
Qi Yang;Haitao Shang;Hui-di Li;H. Xi;Jian-long Wang
通讯作者：
Qi Yang;Haitao Shang;Hui-di Li;H. Xi;Jian-long Wang
DOI：
10.1271/bbb1961.45.63
发表时间：
1981-07
期刊：
Agricultural and biological chemistry
影响因子：
--
作者：
K. Sakai;M. Morita;N. Hamada;Yasuto Watanabe
通讯作者：
K. Sakai;M. Morita;N. Hamada;Yasuto Watanabe
DOI：
10.1271/bbb1961.50.989
发表时间：
1986-08
期刊：
Agricultural and biological chemistry
影响因子：
--
作者：
K. Sakai;N. Hamada;Yasuto Watanabe
通讯作者：
K. Sakai;N. Hamada;Yasuto Watanabe
DOI：
10.1080/00021369.1986.10867494
发表时间：
1986-04
期刊：
Agricultural and biological chemistry
影响因子：
--
作者：
K. Sakai;N. Hamada;Yasuto Watanabe
通讯作者：
K. Sakai;N. Hamada;Yasuto Watanabe