Linkage between dendritic and T cell commitments in human circulating hematopoietic progenitors.
Linkage between dendritic and T cell commitments in human circulating hematopoietic progenitors.
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DOI：
10.4049/jimmunol.1303260
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发表时间：
2014-06-15
期刊：
影响因子：
--
通讯作者：
Kusunoki Y
中科院分区：
文献类型：
--
作者：
Kyoizumi S;Kubo Y;Kajimura J;Yoshida K;Hayashi T;Nakachi K;Young LF;Moore MA;van den Brink MR;Kusunoki Y
树突状细胞（DC）和T细胞在人类造血干细胞中的定向之间的关系还没有很好的理解。在这项研究中，我们计数和表征常规DC（cDC）和浆细胞样DC（pDC）前体与T细胞和胸腺衍生类型的NK细胞前体之间的CD 34+造血祖细胞（HPC）在人外周血（PB）循环。通过与表达Notch 1配体的基质细胞共培养的有限稀释分析，发现在健康供体中，HPC中DC的前体频率（PF）与T细胞PF显著相关，但与NK细胞PF无关。克隆分析表明，大多数T/NK-双重和T-单一谱系前体-但只有少数NK-单一谱系前体-与DC后代的产生相关。所有产生DC和T细胞后代的克隆均发现具有单核细胞和/或粒细胞后代，表明DC通过髓样DC途径分化。PB HPC亚群的分析显示，DC和T/NK细胞祖细胞之间的谱系分裂发生在分叉成T细胞谱系和NK细胞谱系之前的阶段。研究结果表明，DC和T细胞承诺之间存在强烈的联系，这可能是印记在循环淋巴启动的多能祖细胞或更上游的HPC。
The relationships between commitments of dendritic cells (DCs) and T cells in human hematopoietic stem cells are not well-understood. In this study, we enumerate and characterize conventional-DC (cDC) and plasmacytoid-DC (pDC) precursors in association with T-cell and thymus-derived types of NK-cell precursors among CD34+ hematopoietic progenitor cells (HPCs) circulating in human peripheral blood (PB). By limiting-dilution analyses using co-culture with stroma cells expressing Notch1 ligand, the precursor frequencies (PFs) of DCs in HPCs were found to significantly correlate with T-cell PFs, but not with NK-cell PFs, among healthy donors. Clonal analyses showed that the majority of T/NK-dual and T-single lineage precursors - but only a minority of NK-single lineage precursors - were associated with the generation of DC progenies. All clones producing both DC and T-cell progenies were found with monocyte and/or granulocyte progenies, suggesting DC differentiation via myeloid DC pathways. Analyses of PB HPC subpopulations revealed that the lineage split between DC and T/NK-cell progenitor occurs at the stage prior to bifurcation into T- and NK-cell lineages. The findings suggest a strong linkage between DC and T-cell commitments, which may be imprinted in circulating lymphoid-primed multipotent progenitors or in more upstream HPCs.
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DOI：
10.4049/jimmunol.1203189
发表时间：
2013-06-15
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Kyoizumi S;Kubo Y;Kajimura J;Yoshida K;Imai K;Hayashi T;Nakachi K;Young LF;Moore MA;van den Brink MR;Kusunoki Y
通讯作者：
Kusunoki Y
胸腺归巢外周树突状细胞构成稳态胸腺中树突状细胞的三个主要亚群中的两个。
影响因子：
15.3
作者：
Li, JiChu;Park, JooHung;Foss, Deborah;Goldschneider, Irving
通讯作者：
Goldschneider, Irving
DOI：
10.1084/jem.20062648
发表时间：
2007-07-09
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Caton ML;Smith-Raska MR;Reizis B
通讯作者：
Reizis B
影响因子：
5.4
作者：
Luche, Herve;Ardouin, Laurence;Malissen, Bernard
通讯作者：
Malissen, Bernard
影响因子：
64.8
作者：
ARDAVIN, C;WU, L;SHORTMAN, K
通讯作者：
SHORTMAN, K