Basic concepts and methodologies of DNA marker systems in plant molecular breeding.
Basic concepts and methodologies of DNA marker systems in plant molecular breeding.
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DOI：
10.1016/j.heliyon.2021.e08093
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发表时间：
2021-10
期刊：
影响因子：
4
通讯作者：
Amiteye S
中科院分区：
文献类型：
--
作者：
Amiteye S
介绍了植物科学中常用的一些主要分子或 DNA 标记的概念、方法和应用。通过详细解释与标记应用相关的一些值得注意的基本概念，阐明了分子标记技术的一般原理：标记多态性、显性或共显性遗传模式、农艺性状-标记连锁、遗传突变和变异。已广泛综述的分子标记方法有 RFLP、RAPD、SCAR、AFLP、SSR、CpSSR、ISSR、RAMP、SAMPL、SRAP、SSCP、CAPS、SNP、DArT、EST 和 STS。此外，还讨论了基于逆转录转座子的标记方法 IRAP、REMAP、RBIP 和 IPBS 的实用性。此外，还比较了DNA标记的一些显着特征，并将各种标记系统分类为基于PCR或非PCR、显性遗传或共显性遗传、基因座特异性或非特异性以及标记多态性和标记再现性效率的水平。此外，重点介绍了以下DNA标记的原理和方法：五引物扩增阻滞突变系统（PARMS）、保守DNA衍生多态性（CDDP）、基于P450的类似物（PBA）标记、基于微管蛋白的多态性（TBP）、间SINE扩增多态性（ISAP）、序列特异性扩增多态性（S-SAP）、内含子长度多态性（ILP）、间小RNA多态性(iSNAP)、长度多态性直接扩增(DALP)、启动子锚定扩增多态性(PAAP)、目标区域扩增多态性(TRAP)、保守区扩增多态性(CoRAP)、起始密码子靶向(SCoT)多态性和小卫星DNA定向扩增(DAMD)。还概述了最近用于实现植物研究各种目标的一些分子标记应用。这篇综述将为植物育种家和其他科学家以及需要分子或 DNA 标记技术使用基本知识的技术人员和学生提供有用的参考资源。分子标记；植物遗传多样性；多态性；基本概念；基因突变
The concepts, methodologies and applications of some of the major molecular or DNA markers commonly used in plant science have been presented. The general principles of molecular marker techniques have been elucidated with detailed explanation of some notable basic concepts associated with marker applications: marker polymorphism, dominant or co-dominant mode of inheritance, agronomic trait-marker linkage, genetic mutations and variation. The molecular marker methods that have been extensively reviewed are RFLP, RAPD, SCAR, AFLP, SSR, CpSSR, ISSR, RAMP, SAMPL, SRAP, SSCP, CAPS, SNP, DArT, EST, and STS. In addition, the practicality of the retrotransposon-based marker methods, IRAP, REMAP, RBIP, and IPBS, have been discussed. Moreover, some salient characteristics of DNA markers have been compared and the various marker systems classified as PCR- or non-PCR-based, dominantly or co-dominantly inherited, locus specific or non-specific as well as at the levels of marker polymorphism and efficiency of marker reproducibility. Furthermore, the principles and methods of the following DNA markers have been highlighted: Penta-primer amplification refractory mutation system (PARMS), Conserved DNA-Derived Polymorphism (CDDP), P450-based analogue (PBA) markers, Tubulin-Based Polymorphism (TBP), Inter-SINE amplified polymorphism (ISAP), Sequence specific amplified polymorphism (S-SAP), Intron length polymorphisms (ILPs), Inter small RNA polymorphism (iSNAP), Direct amplification of length polymorphisms (DALP), Promoter anchored amplified polymorphism (PAAP), Target region amplification polymorphism (TRAP), Conserved region amplification polymorphism (CoRAP), Start Codon Targeted (SCoT) Polymorphism, and Directed Amplification of Minisatellite DNA (DAMD). Some molecular marker applications that have been recently employed to achieve various objectives in plant research have also been outlined. This review will serve as a useful reference resource for plant breeders and other scientists, as well as technicians and students who require basic know-how in the use of molecular or DNA marker technologies. Molecular marker; Plant genetic diversity; Polymorphism; Basic concept; Genetic mutation
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影响因子：
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作者：
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