Mechanism of cellular oxidation stress induced by asymmetric dimethylarginine.
Mechanism of cellular oxidation stress induced by asymmetric dimethylarginine.
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DOI：
10.3390/ijms13067521
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发表时间：
2012
影响因子：
5.6
通讯作者：
Fung HL
中科院分区：
文献类型：
--
作者：
Mohan S;Fung HL
不对称二甲基精氨酸（ADMA）诱导血管氧化应激的机制尚不清楚。在这项研究中，我们利用人脐静脉内皮细胞 (HUVEC) 来研究 ADMA 细胞转运和内皮一氧化氮合酶 (eNOS) 解偶联在导致这种现象中的作用。二氢乙锭（DHE）荧光被用作氧化应激的指标。当 ADMA 浓度大于 10 μM 时，全细胞及其分离的膜组分表现出可测量的 DHE 荧光增强。 ADMA 诱导的 DHE 荧光可通过与 L-赖氨酸、四氢生物蝶呤 (BH4) 或 L-硝基精氨酸甲酯 (L-NAME) 共孵育来抑制。血管紧张素 II (Ang II) 在这些细胞中诱导的氧化应激不受相同浓度的 L-赖氨酸、L-NAME 和 BH4 的影响。当 BH4 浓度增加时，ADMA 诱导的细胞亚硝酸盐或亚硝酸盐/硝酸盐生成量的减少被逆转。这些结果表明，ADMA 诱导的 DHE 荧光涉及细胞膜中的阳离子转运系统和 eNOS 的参与，而不是 Ang II-NADPH 氧化酶途径的参与。
The mechanism by which asymmetric dimethylarginine (ADMA) induces vascular oxidative stress is not well understood. In this study, we utilized human umbilical vein endothelial cells (HUVEC) to examine the roles of ADMA cellular transport and the uncoupling of endothelial nitric oxide synthase (eNOS) in contributing to this phenomenon. Dihydroethidium (DHE) fluorescence was used as an index of oxidative stress. Whole cells and their isolated membrane fractions exhibited measureable increased DHE fluorescence at ADMA concentrations greater than 10 μM. ADMA-induced DHE fluorescence was inhibited by co-incubation with L-lysine, tetrahydrobiopterin (BH4), or L-nitroarginine methyl ester (L-NAME). Oxidative stress induced in these cells by angiotensin II (Ang II) were unaffected by the same concentrations of L-lysine, L-NAME and BH4. ADMA-induced reduction in cellular nitrite or nitrite/nitrate production was reversed in the presence of increasing concentrations of BH4. These results suggest that ADMA-induced DHE fluorescence involves the participation of both the cationic transport system in the cellular membrane and eNOS instead of the Ang II-NADPH oxidase pathway.
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影响因子：
37.8
作者：
Boger, RH;BodeBoger, SM;Frolich, JC
通讯作者：
Frolich, JC
影响因子：
37.8
作者：
Antoniades, Charalambos;Shirodaria, Cheerag;Channon, Keith M.
通讯作者：
Channon, Keith M.
影响因子：
9.3
作者：
Teerlink, Tom;Luo, Zaiming;Palm, Fredrik;Wilcox, Christopher S.
通讯作者：
Wilcox, Christopher S.
影响因子：
4.1
作者：
MCDERMOTT, JR
通讯作者：
MCDERMOTT, JR
影响因子：
168.9
作者：
Valkonen, VP;Päivä, H;Laaksonen, R
通讯作者：
Laaksonen, R