The C. elegans sex determination gene laf-1 encodes a putative DEAD-box RNA helicase.
The C. elegans sex determination gene laf-1 encodes a putative DEAD-box RNA helicase.
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DOI：
10.1016/j.ydbio.2009.04.003
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发表时间：
2009-06-15
影响因子：
2.7
通讯作者：
Anderson, Philip
中科院分区：
文献类型：
--
作者：
Hubert, Amy;Anderson, Philip
关键词：
梭线虫基因laf-1对胚胎发育和性别决定都至关重要。Laf-1被认为是通过负调节tra-2在雌雄同体和雄性中的表达来促进雄性细胞的命运。我们克隆了laf-1，并确定它编码一个与S.酿酒酵母Ded 1 p和果蝇Vasa。三个测序的laf-1突变是错义等位基因，影响解旋酶基序III中或附近的蛋白质小区域。我们证明了laf-1突变导致的表型是由于laf-1功能的丧失或减少，并且laf-1和相关的解旋酶vbh-1在生殖系性别决定中发挥作用。Laf-1 mRNA在雄性和雌雄同体中以及在雌雄同体的生殖系和索马体中均表达。它在所有发育阶段都有表达，在胚胎中最丰富。LAF-1主要是，如果不是排他的，细胞质和共定位与PGL-1在P颗粒的生殖系前体细胞。以前的结果表明，laf-1的功能，负调控性别决定蛋白TRA-2的表达，我们发现，TRA-2的丰度是适度的laf-1/+女性升高。我们讨论了LAF-1作为解旋酶的潜在功能及其在性别决定中的作用。
The C. elegans gene laf-1 is critical for both embryonic development and sex determination. Laf-1 is thought to promote male cell fates by negatively regulating expression of tra-2 in both hermaphrodites and males. We cloned laf-1 and established that it encodes a putative DEAD-box RNA helicase related to S. cerevisiae Ded1p and Drosophila Vasa. Three sequenced laf-1 mutations are missense alleles affecting a small region of the protein in or near helicase motif III. We demonstrate that the phenotypes resulting from laf-1 mutations are due to loss or reduction of laf-1 function, and that both laf-1 and a related helicase vbh-1 function in germline sex determination. Laf-1 mRNA is expressed in both males and hermaphrodites and in both the germ line and soma of hermaphrodites. It is expressed at all developmental stages and is most abundant in embryos. LAF-1 is predominantly, if not exclusively, cytoplasmic and colocalizes with PGL-1 in P granules of germline precursor cells. Previous results suggest that laf-1 functions to negatively regulate expression of the sex determination protein TRA-2, and we find that the abundance of TRA-2 is modestly elevated in laf-1/+ females. We discuss potential functions of LAF-1 as a helicase and its roles in sex determination.
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