MUC5AC expression in human middle ear epithelium of patients with otitis media.
MUC5AC expression in human middle ear epithelium of patients with otitis media.
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DOI：
10.1001/archoto.2010.123
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发表时间：
2010-08
影响因子：
--
通讯作者：
Papsin, Blake C.
中科院分区：
文献类型：
--
作者：
Kerschner, Joseph E.;Tripathi, Shivani;Khampang, Pawjai;Papsin, Blake C.
粘蛋白基因5AC（MUC 5AC）是中耳中的一种主要分泌型粘蛋白。MUC 5AC在ME粘液样渗出、听力损失的发展中至关重要，并且还提供ME粘膜保护和细菌清除。本研究的目的是比较中耳炎（OM）患者与非OM患者的ME MUC 5AC表达水平。将9个月至7岁的患者接受常规鼓膜造孔管（TT）插入治疗复发性中耳炎（RecOM）或慢性渗出性中耳炎（COME），并与无中耳炎病史的对照患者进行人工耳蜗植入术进行比较。在常规TT放置或人工耳蜗植入期间，获得ME上皮的1 mm活检。提取RNA，并使用真实的时间RT-PCR来定量MUC 5AC表达水平。23例OM患者（12例RecOM和11例COME）使用5个对照进行分析。组间平均年龄无差异。在RecOM样品中，MUC 5AC的平均表达是对照的25.92倍。在COME样品中，平均表达是对照的155.40倍。与对照相比，在RecOM和COME患者中，人ME中MUC 5AC表达的水平显著增加。这项研究证明了OM患者MUC 5AC基因的变化，并强调了需要更好地了解OM的分子反应，特别是粘蛋白。对这些因素的彻底探索将为开发针对OM这一极其常见问题的新干预措施提供机会。
Mucin gene 5AC (MUC5AC) has been identified as a major secretory mucin in the middle ear (ME). MUC5AC is fundamentally important in the development of ME mucoid effusions, hearing loss and also provides ME mucosal protection and bacterial clearance. The objective of this study is to compare levels of ME MUC5AC expression in patients with otitis media (OM) to patients without OM. Patients from 9 months to 7 years undergoing routine tympanostomy tube (TT) insertion for recurrent otitis media (RecOM) or chronic otitis media with effusion (COME) were compared to control patients without a history of otitis media undergoing cochlear implantation. During routine TT placement or cochlear implantation, a 1-mm biopsy of the ME epithelium was obtained. RNA was extracted, and real time RT-PCR was used to quantify levels of MUC5AC expression. Twenty-three OM patients (12 RecOM and 11 COME) were analyzed using 5 controls. Mean age was not different between groups. In RecOM samples, mean expression of MUC5AC was 25.92 times greater than controls. In COME samples, mean expression was 155.40 times greater than controls. Levels of MUC5AC expression in the human ME are significantly increased in both RecOM and COME patients compared to controls. This study demonstrates MUC5AC gene changes in patients with OM and highlights the need for greater understanding of the molecular responses in OM; particularly that of mucin. A thorough exploration of these factors will provide opportunities to develop novel interventions for the extremely common problem of OM.
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作者：
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