Screening of Microbes Associated With Swine Growth and Fat Deposition Traits Across the Intestinal Tract.
Screening of Microbes Associated With Swine Growth and Fat Deposition Traits Across the Intestinal Tract.
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筛选与猪肠道生长和脂肪沉积特性相关的微生物
DOI：
10.3389/fmicb.2020.586776
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发表时间：
2020
影响因子：
5.2
通讯作者：
Cao J
中科院分区：
文献类型：
--
作者：
Tang S;Xin Y;Ma Y;Xu X;Zhao S;Cao J
猪是世界上最常见的牲畜品种之一，预计其生长速度快，皮下脂肪较低，但肌内脂肪较高（“大理石花纹肉”）。如今，人们相信，不仅宿主遗传学，而且其肠道微生物组都可以调节农场动物的表型，然而，许多机制仍然难以捉摸。我们测量了 91 头 260 日龄恩施猪的体重 (BW)、平均日增重 (ADG)、背膘厚度 (BFT) 和肌内脂肪 (IMF)，然后使用 50K 单核苷酸多态性芯片对每头猪进行基因分型，并对来自空肠、回肠、盲肠、结肠和直肠的 455 个微生物样本进行 16S 核糖体 RNA 基因测序。整个肠道的微生物多样性表现出显着的空间差异，其中盲肠和结肠的 α 多样性最高。盲肠和结肠微生物群对 BW 和 ADG 的贡献更大，占表型变异的 22-37%。空肠和盲肠微生物群对 BFT 和 IMF 的贡献比其他部分更多（13-31%）。最后，我们从盲肠、结肠和空肠中鉴定出八个与目标性状显着相关的微生物类群。 Alloprevotella属和Ruminococcaceae UCG-005属与BW和ADG呈高度正相关。盲肠中的Prevotellaceae UCG-001属和Alistipes属以及空肠中的Clostridium sensu stricto 1属与BFT和IMF高度正相关。寡养单胞菌属、球毛菌属和脱硫弧菌属与上述性状呈负相关。这些发现可能有助于制定控制肠道微生物群以改变猪生产性能的策略。
Pigs, as one of the most common livestock species worldwide, are expected to have a fast growth rate and lower subcutaneous fatness but higher intramuscular fat (“marbling meat”). Nowadays, it is believed that not only host genetics but also its gut microbiomes can modulate farm animal phenotypes, however, many of the mechanisms remain elusive. We measured the body weight (BW), average daily gain (ADG), backfat thickness (BFT), and intramuscular fatness (IMF) of 91 Enshi pigs at 260 days of age, then genotyped each one individually using a 50K single nucleotide polymorphism array and performed 16S ribosomal RNA gene sequencing on 455 microbial samples from the jejunum, ileum, cecum, colon, and rectum. The microbial diversity showed notable spatial variation across the entire intestinal tract, with the cecum and colon having the highest α-diversity. The cecal and colonic microbiotas made greater contributions to BW and ADG and accounted for 22–37% of the phenotypic variance. The jejunal and cecal microbiotas contributed more (13–31%) to the BFT and IMF than the other segments. Finally, from cecum, colon, and jejunum, we identified eight microbial taxa that were significantly correlated with the target traits. The genera Alloprevotella and Ruminococcaceae UCG-005 were highly positively correlated with BW and ADG. The genera Prevotellaceae UCG-001 and Alistipes in the cecum and Clostridium sensu stricto 1 in the jejunum were highly positively correlated with BFT and IMF. The genera Stenotrophomonas, Sphaerochaeta, and Desulfovibrio were negatively associated with the mentioned traits. These findings could aid in developing strategies for manipulating the gut microbiota to alter production performance in pigs.
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影响因子：
15.5
作者：
Lu D;Tiezzi F;Schillebeeckx C;McNulty NP;Schwab C;Shull C;Maltecca C
通讯作者：
Maltecca C
影响因子：
3.3
作者：
Camarinha-Silva, Amelia;Maushammer, Maria;Bennewitz, Joern
通讯作者：
Bennewitz, Joern
基于 16S rRNA 基因的关联研究确定了与粪便和盲肠腔中猪肉肌内脂肪含量相关的微生物分类群。
影响因子：
4.2
作者：
Fang S;Xiong X;Su Y;Huang L;Chen C
通讯作者：
Chen C
影响因子：
64.8
作者：
Rothschild, Daphna;Weissbrod, Omer;Segal, Eran
通讯作者：
Segal, Eran
影响因子：
6.4
作者：
Holman DB;Brunelle BW;Trachsel J;Allen HK
通讯作者：
Allen HK