Evidence for the regulation of exocytic transport by protein phosphorylation.
Evidence for the regulation of exocytic transport by protein phosphorylation.
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DOI：
10.1083/jcb.116.6.1343
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发表时间：
1992-03
期刊：
影响因子：
--
通讯作者：
Balch WE
中科院分区：
文献类型：
--
作者：
Davidson HW;McGowan CH;Balch WE
我们研究了蛋白磷酸酶抑制剂冈田酸和微囊藻毒素-LR对新合成的蛋白质通过胞外途径运输的影响。用1 μ M冈田酸处理CHO细胞，可迅速抑制标记蛋白（水泡性口炎病毒G蛋白）从内质网向高尔基体区室的移动。冈田酸和微囊藻毒素-LR也抑制运输在体外试验中重建运动到高尔基室，在浓度相当于那些需要抑制磷酸化酶磷酸酶活性。蛋白激酶抑制剂可拮抗体内和体外的抑制作用，这表明蛋白磷酸化是这种作用的直接原因。与囊泡形成或靶向相关的运输反应的早期阶段被蛋白磷酸化抑制，这可以被富含蛋白磷酸酶2A的组分逆转。蛋白激酶拮抗剂并没有抑制运输之间的顺序隔室的胞吐途径在体外，这表明蛋白磷酸化本身并不需要囊泡运输。在有丝分裂过程中，泡运输被抑制的同时，促成熟因子的激活。有人提出，冈田酸和微囊藻毒素-LR引起的抑制涉及一个类似的机制，负责有丝分裂阻滞的囊泡运输。
We investigated the effects of the protein phosphatase inhibitors okadaic acid and microcystin-LR upon transport of newly synthesized proteins through the exocytic pathway. Treatment of CHO cells with 1 microM okadaic acid rapidly inhibited movement of a marker protein (vesicular stomatitis virus G protein) from the endoplasmic reticulum to the Golgi compartment. Both okadaic acid and microcystin-LR also inhibited transport in an in vitro assay reconstituting movement to the Golgi compartment, at concentrations equivalent to those required to inhibit phosphorylase phosphatase activity. Inhibition both in vivo and in vitro could be antagonized by protein kinase inhibitors, suggesting that protein phosphorylation was directly responsible for this effect. An early stage in the transport reaction associated with vesicle formation or targeting was inhibited by protein phosphorylation, which could be reversed by fractions enriched in protein phosphatase 2A. Protein kinase antagonists did not inhibit transport between sequential compartments of the exocytic pathway in vitro, suggesting that protein phosphorylation is not itself required for vesicular transport. During mitosis, vesicular transport is inhibited simultaneous to the activation of maturation-promoting factor. It is proposed that the inhibition caused by okadaic acid and microcystin-LR involves a similar mechanism to that responsible for the mitotic arrest of vesicular transport.
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DOI：
10.1083/jcb.101.6.2036
发表时间：
1985-12
期刊：
The Journal of cell biology
影响因子：
--
作者：
Featherstone C;Griffiths G;Warren G
通讯作者：
Warren G
影响因子：
11.4
作者：
FELIX, MA;COHEN, P;KARSENTI, E
通讯作者：
KARSENTI, E
影响因子：
3.5
作者：
COHEN, PTW;BREWIS, ND;MANN, DJ
通讯作者：
MANN, DJ
影响因子：
3.8
作者：
FLAMAND, A
通讯作者：
FLAMAND, A
影响因子：
2.9
作者：
FREEMAN, RS;DONOGHUE, DJ
通讯作者：
DONOGHUE, DJ