Photopolymerized microfeatures for directed spiral ganglion neurite and Schwann cell growth.
Photopolymerized microfeatures for directed spiral ganglion neurite and Schwann cell growth.
复制标题
DOI：
10.1016/j.biomaterials.2012.09.053
复制
发表时间：
2013-01
期刊：
影响因子：
14
通讯作者：
Guymon, C. Allan
中科院分区：
文献类型：
--
作者：
Tuft, Bradley W.;Li, Shufeng;Xu, Linjing;Clarke, Joseph C.;White, Scott P.;Guymon, Bradley A.;Perez, Krystian X.;Hansen, Marlan R.;Guymon, C. Allan
人工耳蜗（CIs）为重度听力障碍患者提供听觉感知。然而，它们编码复杂听觉刺激的能力受到限制，部分原因是内耳内电流扩散造成的空间分辨率差。将神经细胞过程导向目标电极可以减少有问题的电流扩散并提高刺激特异性。在这项工作中，光聚合被用于制造微和纳米模式的甲基丙烯酸酯聚合物，以探测基于底物地形线索变化的螺旋神经节神经元（SGN）神经突和雪旺细胞（SGSC）接触引导的程度。利用具有平行线空间光栅，周期为10 - 100 μm的掩膜选择性地阻挡光线，通过快速的单步反应形成微图纹衬底。通过调节紫外曝光时间、光强和光引发剂浓度，可产生250 nm ~ 10 μm的通道振幅。利用扫描电子和原子力显微镜观察到脊线和凹槽之间的逐渐转变。这些过渡与蚀刻光刻技术产生的垂直特征形成鲜明对比。神经单元的对齐随特征幅值的增大和周期的恒定而显著增加，随特征幅值的减小和周期的恒定而显著增加。SGN神经突排列与最大特征斜率密切相关（r = 0.93）。多种神经元和神经胶质类型以不同程度的对齐方式定向。这项工作提出了一种方法来制造逐渐倾斜的微模式，用于细胞接触引导研究，并展示了内耳神经元件响应微纳米级表面形貌的空间控制。
Cochlear implants (CIs) provide auditory perception to individuals with severe hearing impairment. However, their ability to encode complex auditory stimuli is limited due, in part, to poor spatial resolution caused by electrical current spread in the inner ear. Directing nerve cell processes towards target electrodes may reduce the problematic current spread and improve stimulatory specificity. In this work, photopolymerization was used to fabricate micro- and nano-patterned methacrylate polymers to probe the extent of spiral ganglion neuron (SGN) neurite and Schwann cell (SGSC) contact guidance based on variations in substrate topographical cues. Micropatterned substrates are formed in a rapid, single-step reaction by selectively blocking light with photomasks which have parallel line-space gratings with periodicities of 10 – 100 μm. Channel amplitudes of 250 nm – 10 μm are generated by modulating UV exposure time, light intensity, and photoinitiator concentration. Gradual transitions are observed between ridges and grooves using scanning electron and atomic force microscopy. The transitions stand in contrast to vertical features generated via etching lithographic techniques. Alignment of neural elements increases significantly with increasing feature amplitude and constant periodicity, as well as with decreasing periodicity and constant amplitude. SGN neurite alignment strongly correlates (r = 0.93) with maximum feature slope. Multiple neuronal and glial types orient to the patterns with varying degrees of alignment. This work presents a method to fabricate gradually-sloping micropatterns for cellular contact guidance studies and demonstrates spatial control of inner ear neural elements in response to micro- and nano-scale surface topography.
登录
查看更多内容
影响因子：
2.8
作者：
Clarke, Joseph C.;Tuft, Bradley W.;Clinger, John D.;Levine, Rachel;Figueroa, Lucas Sievens;Guymon, C. Allan;Hansen, Marlan R.
通讯作者：
Hansen, Marlan R.
影响因子：
14
作者：
Bryant, Stephanie J.;Cuy, Janet L.;Ratner, Buddy D.
通讯作者：
Ratner, Buddy D.
影响因子：
14
作者：
Branch, DW;Wheeler, BC;Leckband, DE
通讯作者：
Leckband, DE
影响因子：
14
作者：
Charest, JL;Eliason, MT;King, WP
通讯作者：
King, WP
影响因子：
14
作者：
Johansson, F;Carlberg, P;Kanje, M
通讯作者：
Kanje, M