Co-localization of CENP-C and CENP-H to discontinuous domains of CENP-A chromatin at human neocentromeres.
Co-localization of CENP-C and CENP-H to discontinuous domains of CENP-A chromatin at human neocentromeres.
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DOI：
10.1186/gb-2007-8-7-r148
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发表时间：
2007
期刊：
影响因子：
12.3
通讯作者：
Warburton, Peter E.
中科院分区：
文献类型：
--
作者：
Alonso, Alicia;Fritz, Bjoern;Hasson, Dan;Abrusan, Gyoergy;Cheung, Fanny;Yoda, Kinya;Radlwimmer, Bernhard;Ladurner, Andreas G.;Warburton, Peter E.
着丝粒染色质相关蛋白在人类新着丝粒DNA中的分布表明，该染色质由几个含CENP-A的子域组成。哺乳动物着丝粒的形成依赖于含有着丝粒蛋白（CENP）-A的染色质，CENP-A是着丝粒特异性组蛋白H3变体。人类新着丝粒细胞在低拷贝复杂DNA上的异位染色体位置表观遗传地获得CENP-A染色质。新着丝粒允许详细调查着丝粒染色质组织，这是不可能的高度重复的α卫星DNA存在于内源性着丝粒。我们已经检查了CENP-A的分布，以及两个额外的着丝粒染色质相关蛋白（CENP-C和CENP-H），在新着丝粒DNA上使用染色质免疫沉淀（ChIP）在三种不同分辨率的定制基因组微阵列上进行CHIP检测。使用跨越条带13q31.3至13q33.1的连续细菌人工染色体（BAC）微阵列分析两个新着丝粒显示CENP-C和CENP-H共定位于CENP-A染色质结构域。使用更高分辨率的聚合酶链反应（PCR）-扩增子微阵列跨越新着丝粒，我们发现，CENP-A染色质是不连续的，由约87.8个酶（kb）的主要结构域和约13.2 kb的次要结构域组成，由约158 kb的区域没有CENPs分开。两个CENP-A结构域都表现出CENP-C和CENP-H的共定位，定义了一个独特的内部动粒染色质结构，这与着丝粒处的高阶染色质成环模型一致。PCR微阵列数据表明CENP-A核小体在主要结构域中的密度不同，这使用更高分辨率的基于寡核苷酸的微阵列进行了证实。着丝粒染色质由几个CENP-A亚结构域组成，在单个核小体水平和更高级的染色质水平上具有高度不连续的CENP-A染色质，这引起了关于着丝粒染色质整体结构的问题。
The distribution of centromeric chromatin-associated proteins across human neocentromeric DNA shows that this chromatin consists of several CENP-A-containing sub-domains. Mammalian centromere formation is dependent on chromatin that contains centromere protein (CENP)-A, which is the centromere-specific histone H3 variant. Human neocentromeres have acquired CENP-A chromatin epigenetically in ectopic chromosomal locations on low-copy complex DNA. Neocentromeres permit detailed investigation of centromeric chromatin organization that is not possible in the highly repetitive alpha satellite DNA present at endogenous centromeres. We have examined the distribution of CENP-A, as well as two additional centromeric chromatin-associated proteins (CENP-C and CENP-H), across neocentromeric DNA using chromatin immunoprecipitation (ChIP) on CHIP assays on custom genomic microarrays at three different resolutions. Analysis of two neocentromeres using a contiguous bacterial artificial chromosome (BAC) microarray spanning bands 13q31.3 to 13q33.1 shows that both CENP-C and CENP-H co-localize to the CENP-A chromatin domain. Using a higher resolution polymerase chain reaction (PCR)-amplicon microarray spanning the neocentromere, we find that the CENP-A chromatin is discontinuous, consisting of a major domain of about 87.8 kilobases (kb) and a minor domain of about 13.2 kb, separated by an approximately 158 kb region devoid of CENPs. Both CENP-A domains exhibit co-localization of CENP-C and CENP-H, defining a distinct inner kinetochore chromatin structure that is consistent with higher order chromatin looping models at centromeres. The PCR microarray data suggested varying density of CENP-A nucleosomes across the major domain, which was confirmed using a higher resolution oligo-based microarray. Centromeric chromatin consists of several CENP-A subdomains with highly discontinuous CENP-A chromatin at both the level of individual nucleosomes and at higher order chromatin levels, raising questions regarding the overall structure of centromeric chromatin.
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影响因子：
11.4
作者：
Fukagawa, T;Mikami, Y;Ikemura, T
通讯作者：
Ikemura, T
影响因子：
7.8
作者：
Jansen, Lars E. T.;Black, Ben E.;Foltz, Daniel R.;Cleveland, Don W.
通讯作者：
Cleveland, Don W.
DOI：
10.1083/jcb.200210005
发表时间：
2003-01-06
期刊：
The Journal of cell biology
影响因子：
--
作者：
Goshima G;Kiyomitsu T;Yoda K;Yanagida M
通讯作者：
Yanagida M
影响因子：
64.8
作者：
Buchwitz, BJ;Ahmad, K;Henikoff, S
通讯作者：
Henikoff, S
DOI：
10.1073/pnas.97.3.1148
发表时间：
2000-02-01
影响因子：
11.1
作者：
Howman, EV;Fowler, KJ;Choo, KHA
通讯作者：
Choo, KHA