Simultaneous measurement of membrane potential changes in multiple pattern generating neurons using voltage sensitive dye imaging
Simultaneous measurement of membrane potential changes in multiple pattern generating neurons using voltage sensitive dye imaging
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使用电压敏感染料成像同时测量多种模式生成神经元的膜电位变化
DOI：
10.1016/j.jneumeth.2011.09.015
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发表时间：
2012
影响因子：
3
通讯作者：
Andras
中科院分区：
文献类型：
--
作者：
Städele;Andras
利用电压敏感染料（VSD）的光学成像是一种很有前途的技术，用于同时记录许多单个神经元的活动。虽然这种同步记录对于理解神经系统的整体功能至关重要，但如果不了解底层电路的连接性，则难以在单个神经元水平上进行功能解释。中央模式生成电路，如幽门和胃磨电路在口胃神经节（STG）的甲壳类动物，允许这样的调查，由于他们众所周知的连接性，并已作出了很大贡献，我们了解一般的神经元机制。在这里，我们提出了第一次同时光学记录的模式产生的神经元在STG的两个甲壳类动物的物种使用批量加载的VSD二-4-ANEPPS。我们证明了记录的放电活动和突触的相互作用的电路神经元，以及电路间的相互作用，在其功能的背景下，即没有人工刺激。使用简单的事件触发平均可以唯一地识别神经元。我们在两种不同的甲壳类动物（龙虾和螃蟹）中测试了这项技术，因为有几种甲壳类动物被用于研究运动模式的产生。光信号的信噪比在两个物种中都足够高，以导出网络神经元之间的相位关系，以及动作电位和兴奋性和抑制性突触后电位。我们认为，神经网络的成像与可识别的神经元与众所周知的连接，如在STG，是至关重要的网络功能的出现的理解。
Optical imaging using voltage-sensitive dyes (VSDs) is a promising technique for the simultaneous activity recording of many individual neurons. While such simultaneous recordings are critical for the understanding of the integral functionality of neural systems, functional interpretations on a single neuron level are difficult without knowledge of the connectivity of the underlying circuit. Central pattern generating circuits, such as the pyloric and gastric mill circuits in the stomatogastric ganglion (STG) of crustaceans, allow such investigations due to their well-known connectivities and have already contributed much to our understanding of general neuronal mechanisms. Here we present for the first time simultaneous optical recordings of the pattern generating neurons in the STG of two crustacean species using bulk loading of the VSD di-4-ANEPPS. We demonstrate the recording of firing activities and synaptic interactions of the circuit neurons as well as inter-circuit interactions in their functional context, i.e. without artificial stimulation. Neurons could be uniquely identified using simple event-triggered averaging. We tested this technique in two different species of crustaceans (lobsters and crabs), since several crustacean species are used for studying motor pattern generation. The signal-to-noise ratio of the optical signal was high enough in both species to derive phase-relationship between the network neurons, as well as action potentials and excitatory and inhibitory postsynaptic potentials. We argue that imaging of neural networks with identifiable neurons with well-known connectivity, like in the STG, is crucial for the understanding of emergence of network functionality.
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影响因子：
12.8
作者：
Yoo, Seungbo;Jeong, Yun Hee;Choi, Hong-Hee;Chae, Sehyun;Hwang, Daehee;Shin, Sung Jae;Ha, Sang-Jun
通讯作者：
Ha, Sang-Jun
蟹口胃神经节振荡神经元的空间和时间分辨钙浓度变化。
DOI：
10.1073/pnas.86.5.1679
发表时间：
1989
影响因子：
11.1
作者：
Ross,WN;Graubard,K
通讯作者：
Graubard,K
荧光电压敏感染料对蟹口胃神经节神经元活动的光诱导作用
影响因子：
3
作者：
W. Stein;Péter András
通讯作者：
Péter András
影响因子：
5.7
作者：
J. Ramirez;D. Richter
通讯作者：
J. Ramirez;D. Richter
影响因子：
2.5
作者：
SALZBERG, BM;GRINVALD, A;ROSS, WN
通讯作者：
ROSS, WN