The CRISPR-Cas systems were selectively inactivated during evolution of Bacillus cereus group for adaptation to diverse environments
The CRISPR-Cas systems were selectively inactivated during evolution of Bacillus cereus group for adaptation to diverse environments
复制标题
蜡样芽孢杆菌群进化过程中，CRISPR-Cas系统被选择性失活以适应不同的环境
DOI：
10.1038/s41396-020-0623-5
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发表时间：
2020-03
期刊：
影响因子：
--
通讯作者：
Peng Donghai
中科院分区：
文献类型：
--
作者：
Zheng Ziqiang;Zhang Yulan;Liu Zhiyu;Dong Zhaoxia;Xie Chuanshuai;Bravo Alej;ra;Soberon Mario;Mahillon Jacques;Sun Ming;Peng Donghai
CRISPR-CAS系统被认为是水平基因转移(HGT)的障碍。然而，这些系统对物种内HGT的影响尚不清楚。此外，对CRISPR-Cas系统在种群水平上对细菌进化的影响知之甚少。在这里，我们以蜡状芽孢杆菌为模型，在种群规模上研究CRISPR-Cas系统和HGT之间的相互作用。我们发现只有一小部分菌株具有CRISPR-Cas系统(1871年的13.9%)，根据其基因含量分析，大多数这样的系统是有缺陷的。比较基因组学分析表明，CRISPR-Cas系统是HGT的障碍，因为与没有CRISPR-Cas系统的菌株相比，具有活性系统的菌株含有较少的可移动遗传元件(MGES)，独特基因的比例较低，并且环境分布也有限。在缺乏CRISPR-Cas系统的菌株中引入具有功能的CRISPR-Cas系统，导致转化菌株对各种胁迫的适应性降低，致病性降低，表明蜡样芽孢杆菌组菌株可以从灭活这种系统中受益。我们的工作提供了一个大规模的案例，支持CRISPR-Cas系统是物种内HGT的障碍，并且在蜡样芽孢杆菌组中，CRISPR-Cas系统的失活与获得MGES相关，这可能导致更好地适应不同的环境。
CRISPR-Cas systems are considered as barriers to horizontal gene transfer (HGT). However, the influence of such systems on HGT within species is unclear. Also, little is known about the impact of CRISPR-Cas systems on bacterial evolution at the population level. Here, using Bacillus cereus sensu lato as model, we investigate the interplay between CRISPR-Cas systems and HGT at the population scale. We found that only a small fraction of the strains have CRISPR-Cas systems (13.9% of 1871), and most of such systems are defective based on their gene content analysis. Comparative genomic analysis revealed that the CRISPR-Cas systems are barriers to HGT within this group, since strains harboring active systems contain less mobile genetic elements (MGEs), have lower fraction of unique genes and also display limited environmental distributions than strains without active CRISPR-Cas systems. The introduction of a functional CRISPR-Cas system into a strain lacking the system resulted in reduced adaptability to various stresses and decreased pathogenicity of the transformant strain, indicating that B. cereus group strains could benefit from inactivating such systems. Our work provides a large-scale case to support that the CRISPR-Cas systems are barriers to HGT within species, and that in the B. cereus group the inactivation of CRISPR-Cas systems correlated with acquisition of MGEs that could result in better adaptation to diverse environments.
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影响因子：
56.9
作者：
McDaniel, Lauren D.;Young, Elizabeth;Paul, John H.
通讯作者：
Paul, John H.
影响因子：
14.9
作者：
Grissa, Ibtissem;Vergnaud, Gilles;Pourcel, Christine
通讯作者：
Pourcel, Christine
影响因子：
10.7
作者：
Price MN;Dehal PS;Arkin AP
通讯作者：
Arkin AP
DOI：
10.1126/science.1165771
发表时间：
2008-12-19
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Marraffini LA;Sontheimer EJ
通讯作者：
Sontheimer EJ
影响因子：
28.3
作者：
Turgeman-Grott I;Joseph S;Marton S;Eizenshtein K;Naor A;Soucy SM;Stachler AE;Shalev Y;Zarkor M;Reshef L;Altman-Price N;Marchfelder A;Gophna U
通讯作者：
Gophna U