Not all mice are equal: welfare implications of behavioural habituation profiles in four 129 mouse substrains.
Not all mice are equal: welfare implications of behavioural habituation profiles in four 129 mouse substrains.
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DOI：
10.1371/journal.pone.0042544
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Ohl F
中科院分区：
文献类型：
--
作者：
Boleij H;Salomons AR;van Sprundel M;Arndt SS;Ohl F
在动物实验研究中，保障动物福利是确定饲养和管理标准的一个重要目标。虽然这些标准通常是按动物物种定义的，但已知实验室小鼠品系之间存在相当大的差异，例如关于它们的情感特征。在早期的实验中，我们发现129 P3小鼠在重复暴露于最初的新环境后表现出缺乏焦虑相关行为的习惯化（非适应性特征），我们在此研究了其他四种129近交系小鼠亚系（129 S2/SvPas，129 S2/SvHsd（exp 1）; 129 P2和129 X1（exp 2））对焦虑相关行为习惯化的影响。将每种品系的雄性小鼠重复置于改良的孔板试验中，测量焦虑相关行为、探索行为和运动行为。结果表明，所有四个亚株在整个测试期间均显示出缺乏习惯性行为。虽然不是在所有的亚株的一般活动的可能的混杂效应可以排除，我们的研究结果表明，129个亚株的遗传背景可能会增加他们的脆弱性，以科普环境的挑战，如接触到新奇。与其他品系相比，在标准实验室条件下，这种脆弱性可能会对这些小鼠的福利产生负面影响。根据我们的研究结果，我们建议考虑（子）应变特定的指导方针和协议，考虑（子）列车特定的适应能力。
Safeguarding the welfare of animals is an important aim when defining housing and management standards in animal based, experimental research. While such standards are usually defined per animal species, it is known that considerable differences between laboratory mouse strains exist, for example with regard to their emotional traits. Following earlier experiments, in which we found that 129P3 mice show a lack of habituation of anxiety related behaviour after repeated exposure to an initially novel environment (non-adaptive profile), we here investigated four other 129 inbred mouse substrains (129S2/SvPas, 129S2/SvHsd (exp 1); 129P2 and 129X1 (exp 2)) on habituation of anxiety related behaviour. Male mice of each strain were repeatedly placed in the modified hole board test, measuring anxiety-related behaviour, exploratory and locomotor behaviour. The results reveal that all four substrains show a lack of habituation behaviour throughout the period of testing. Although not in all of the substrains a possible confounding effect of general activity can be excluded, our findings suggest that the genetic background of the 129 substrains may increase their vulnerability to cope with environmental challenges, such as exposure to novelty. This vulnerability might negatively affect the welfare of these mice under standard laboratory conditions when compared with other strains. Based on our findings we suggest to consider (sub)strain-specific guidelines and protocols, taking the (subs)train-specific adaptive capabilities into account.
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DOI：
10.1523/jneurosci.4904-07.2008
发表时间：
2008-08-06
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Hefner K;Whittle N;Juhasz J;Norcross M;Karlsson RM;Saksida LM;Bussey TJ;Singewald N;Holmes A
通讯作者：
Holmes A
影响因子：
2.9
作者：
Montkowski, A;Poettig, M;Holsboer, F
通讯作者：
Holsboer, F
影响因子：
2.5
作者：
Bothe, GWM;Bolivar, VJ;Geistfeld, JG
通讯作者：
Geistfeld, JG
DOI：
10.1111/j.1601-183x.2009.00519.x
发表时间：
2009-11
期刊：
Genes, brain, and behavior
影响因子：
--
作者：
Camp M;Norcross M;Whittle N;Feyder M;D'Hanis W;Yilmazer-Hanke D;Singewald N;Holmes A
通讯作者：
Holmes A
影响因子：
2.5
作者：
Kim, D;Chae, S;Shin, HS
通讯作者：
Shin, HS